Generation of migratory antigen-specific plasma blasts and mobilization of resident plasma cells in a secondary immune response
Generation of migratory antigen-specific plasma blasts and mobilization of resident plasma cells in a secondary immune response
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DOI：
10.1182/blood-2004-07-2507
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发表时间：
2005-02-15
期刊：
影响因子：
20.3
通讯作者：
Dörner, T
中科院分区：
文献类型：
--
作者：
Odendahl, M;Mei, H;Dörner, T
保护性体液免疫的维持依赖于抗体分泌细胞的产生和存活。骨髓为次级淋巴器官中全身免疫应答过程中产生的浆细胞的长期存活提供了小生境。在这里，我们分析了迁移的人血浆母细胞和浆细胞后，二次接种破伤风毒素。在免疫后第6天和第7天，分泌CD 19（+）/CD 27（高）/细胞内免疫球蛋白G（高）（IgG（高））/HLA-DR高/CD 38（高）/CD 20（-）/CD 95（+）破伤风毒素特异性抗体的血浆原始细胞从次级淋巴器官大量释放到血液中。这些细胞对CXCR 3和CXCR 4的配体表现出趋化反应，可能将它们引导到骨髓或发炎组织。同时，血液中大量出现CD 19（+）/CD 27（高）/细胞内IgG（高）/HLA-DR低/CD 38（+）/CD 20（-）/CD 95（+）细胞群。这些细胞具有长寿浆细胞的表型，分泌特异性未知的抗体，而不是破伤风类毒素。这些浆细胞在血液中的出现表明，新产生的浆母细胞和常驻浆细胞之间成功竞争骨髓中的生存小生境，这是建立体液记忆及其可塑性的基本机制。(C)2005年美国血液学会。
Maintenance of protective humoral immunity depends on the generation and survival of antibody-secreting cells. The bone marrow provides niches for long-term survival of plasma cells generated in the course of systemic immune responses in secondary lymphoid organs. Here, we have analyzed migratory human plasma blasts and plasma cells after secondary vaccination with tetanus toxin. On days 6 and 7 after immunization, CD19(+)/CD27(high)/intracellular immunoglobulin G(high) (IgG(high))/HLA-DRhigh/CD38(high)/CD20(-)/CD95(+) tetanus toxin-specific antibody-secreting plasma blasts were released in large numbers from the secondary lymphoid organs into the blood. These cells show chemotactic responsiveness toward ligands for CXCR3 and CXCR4, probably guiding them to the bone marrow or inflamed tissue. At the same time, a population of CD19(+)/CD27(high)/intracellular IgG(high)/HLA-DRlow/CD38(+)/CD20(-)/CD95(+) cells appeared in the blood in large numbers. These cells, with the phenotype of long-lived plasma cells, secreted antibodies of unknown specificity, not tetanus toxoid. The appearance of these plasma cells in the blood indicates successful competition for survival niches in the bone marrow between newly generated plasma blasts and resident plasma cells as a fundamental mechanism for the establishment of humoral memory and its plasticity. (C) 2005 by The American Society of Hematology.