ALTERATIONS OF SINGLE-MOLECULE FLUORESCENCE LIFETIMES IN NEAR-FIELD OPTICAL MICROSCOPY
ALTERATIONS OF SINGLE-MOLECULE FLUORESCENCE LIFETIMES IN NEAR-FIELD OPTICAL MICROSCOPY
复制标题
DOI：
10.1126/science.265.5170.364
复制
发表时间：
1994-07-15
期刊：
影响因子：
56.9
通讯作者：
KELLER, RA
中科院分区：
文献类型：
--
作者：
AMBROSE, WP;GOODWIN, PM;KELLER, RA
采用脉冲激光激发、时间相关单光子计数和近场扫描光学显微镜（NSOM）测量了二氧化硅表面单个罗丹明6G分子的荧光寿命。荧光寿命随分子相对于近场探针的位置而变化。寿命降低的定性特征与金属表面附近分子激发态猝灭效应一致。NSOM技术提供了一种以可重复的方式改变单个荧光分子环境及其衰变动力学的方法。
Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical microscopy (NSOM). The fluorescence lifetime varies with the position of a molecule relative to a near-field probe. Qualitative features of lifetime decreases are consistent with molecular excited state quenching effects near metal surfaces. The technique of NSOM provides a means of altering the environment of a single fluorescent molecule and its decay kinetics in a repeatable fashion.