Pretreatment of methanogenic granules for immobilized hydrogen fermentation
Pretreatment of methanogenic granules for immobilized hydrogen fermentation
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DOI：
10.1016/j.ijhydene.2007.03.005
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发表时间：
2007-10-01
影响因子：
7.2
通讯作者：
Chen, Shulin
中科院分区：
文献类型：
--
作者：
Hu, Bo;Chen, Shulin
氢气可以通过在厌氧条件下的混合细菌培养中发酵糖来产生。提出厌氧颗粒污泥作为固定化产氢菌，在预处理过程中消除颗粒的产甲烷活性后用于氢气发酵。报道了一种利用氯仿直接将产甲烷颗粒转化为产氢颗粒的创新处理方法。比较了氯仿处理与酸处理、热处理处理对污泥和产甲烷颗粒产甲烷活性的抑制效果。结果表明，三种方法中氯仿处理效果最好。酸和热处理是有效的污水污泥处理被观察到不是非常有效的应用于颗粒，因为从颗粒结构的保护。而产甲烷菌对氯仿非常敏感，即使氯仿浓度很低。在培养基中添加0.05%氯仿后，污泥和颗粒的甲烷产量几乎完全被抑制。当氯仿浓度控制在较低水平时，氯仿选择性地抑制了产甲烷活性，而不影响产氢。在高浓度范围内，氯仿对氢气的生成也有抑制作用。氯仿引起不可逆的产甲烷活性消除，但停止氯仿后产氢恢复正常。氯仿对产氢菌产甲烷菌的抑制表现出良好的选择性，几乎永久地消除了甲烷的产生，推迟了氢的消耗到乙酸，同时允许回收正常的产氢。氯仿处理的颗粒重复培养8次，无明显损伤。用氯仿处理过的颗粒连续培养表明，颗粒结构可保持15天以上，10天后开始形成新的颗粒。HRT 5.3h产氢量达到11.6L/L/d，表明氯仿处理产甲烷颗粒在固定化氢气发酵中的应用潜力。(C) 2007年国际氢能协会。Elsevier Ltd.出版。版权所有。
Hydrogen can be produced through fermenting sugars in a mixed bacterial culture under anaerobic conditions. Anaerobic granular sludge was proposed as immobilized hydrogen producing bacteria to be used in hydrogen fermentation after methanogenic activity of the granule was eliminated in the pretreatment process. This paper reports an innovative treatment method to directly convert methanogenic granules to hydrogen producing granules using chloroform. Chloroform treatment was compared against acid and heat treatments of sewage sludge and methanogenic granules in terms of effectiveness to eliminate methanogenic activity. The results showed that chloroform treatment was the most effective among the three methods tested. Acid and heat treatments that were effective for sewage sludge treatment were observed not highly effective for application to granules because of the protection from the granular structure. In contrast, methanogens were very sensitive to the chloroform even at very low level. Methane production was almost completely inhibited in both sewage sludge and granules with the treatment with only 0.05% chloroform addition into the culture medium. If the chloroform concentration was controlled at low levels, chloroform selectively inhibited methanogenic activity while did not affect the hydrogen production. At high concentration range, chloroform also inhibited hydrogen production. Chloroform caused irreversible methanogenic activity elimination but hydrogen production recovered to normal after chloroform addition stopped. Chloroform showed desirable selectivity on inhibition of methanogens from hydrogen producing bacteria, nearly permanently eliminated the methane production, postponed the hydrogen consumption to acetic acid, while allowed recovery for normal hydrogen production. Chloroform treated granules were repeatedly cultured for eight time without noticeable damage. Continuous culture with chloroform treated granules showed that the granule structure could be kept for over 15 days and new granules started to form after 10 days operation. The hydrogen productivity reached 11.6L/L/day at HRT 5.3h, which all showed potential application of chloroform treatment of methanogenic granules in the immobilized hydrogen fermentation. (C) 2007 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.