Angiopoietin 1 and angiopoietin 2 in follicular fluid of women undergoing a long protocol
Angiopoietin 1 and angiopoietin 2 in follicular fluid of women undergoing a long protocol
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DOI：
10.1016/j.fertnstert.2011.09.031
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发表时间：
2011-12-01
影响因子：
6.7
通讯作者：
Kanzaki, Hideharu
中科院分区：
文献类型：
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作者：
Nishigaki, Akemi;Okada, Hidetaka;Kanzaki, Hideharu
目的：测定人个体排卵前卵泡中血管生成素1 （ANGPT1）和ANGPT2的浓度与卵泡直径或体积的关系，以阐明这些分子在卵泡发生中的作用。设计：前瞻性研究。地点：日本大阪关西医科大学研究实验室。患者：23名接受体外受精的女性。干预措施：取卵当天，采集单个卵泡的血清和卵泡液（FF）。我们分析了348个卵泡。主要观察指标：FF中ANGPT1和ANGPT2浓度、血清和卵母细胞回收率。结果：FF中ANGPT1浓度平均比血清低150倍，而ANGPT2浓度平均比血清高8倍。直径为18 mm的卵泡中ANGPT1的浓度。另一方面，直径= 18 mm的卵泡中ANGPT2的浓度。随着卵泡直径增大，ANGPT2/ANGPT1比值增大。FF中ANGPT1浓度随卵泡体积的增大而降低。FF中ANGPT2浓度和ANGPT2/ANGPT1比值随卵泡体积增大而升高。卵母细胞恢复组FF中ANGPT2/ANGPT1比值显著高于未恢复组。结论：我们的数据表明，ANGPT1和ANGPT2水平的变化可能与排卵前卵泡生长和血管生成有关。肥料(R) 2011；96: 1378 - 83。(C) 2011年美国生殖医学学会。)
Objective: To determine the concentrations of angiopoietin 1 (ANGPT1) and ANGPT2 in individual human pre-ovulatory follicles in relation to their diameter or volume to clarify the role of these molecules in folliculogenesis.Design: Prospective study.Setting: Research laboratory at Kansai Medical University, Osaka, Japan.Patient(s): Twenty-three women undergoing IVF.Intervention(s): On the day of oocyte retrieval, serum samples and follicular fluid (FF) from individual follicles were collected. We analyzed 348 follicles.Main Outcome Measure(s): ANGPT1 and ANGPT2 concentrations in FF and serum and oocyte recovery rates.Result(s): On average, ANGPT1 concentrations in FF were 150 times lower than those in serum, whereas ANGPT2 concentrations in FF were 8 times higher than those in serum. The concentrations of ANGPT1 in follicles with a diameter = 18 mm. On the other hand, the concentrations of ANGPT2 in follicles with a diameter = 18 mm. The ANGPT2/ANGPT1 ratio increased with enlargement of follicular diameter. ANGPT1 concentrations in FF decreased with follicular volume. ANGPT2 concentrations and the ANGPT2/ANGPT1 ratio in FF rose with follicular volume. The ANGPT2/ANGPT1 ratio in FF from the oocyte recovery group was significantly higher than that from the nonrecovery group.Conclusion(s): Our data suggested that the change in ANGPT1 and ANGPT2 levels may be associated with follicular growth and angiogenesis during the preovulatory period. (Fertil Steril (R) 2011; 96: 1378-83. (C) 2011 by American Society for Reproductive Medicine.)