Photodynamic Graphene Quantum Dot: Reduction Condition Regulated Photoactivity and Size Dependent Efficacy
Photodynamic Graphene Quantum Dot: Reduction Condition Regulated Photoactivity and Size Dependent Efficacy
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DOI：
10.1021/acsami.5b11154
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发表时间：
2016-02-10
影响因子：
9.5
通讯作者：
Li, Yong Y.
中科院分区：
文献类型：
--
作者：
Du, Dou;Wang, Kun;Li, Yong Y.
光动力疗法（PDT）中非常需要光敏剂（PS）的预猝灭和选择性激活，以避免由于PS的非特异性激活和较差的靶向选择性而导致的脱靶效应。在这项研究中，纳米石墨烯材料作为一种独特的x共轭平面系统用于电子转移，被用作PS临时猝灭的强大平台。通过还原可裂解二硫键将光敏剂二氢卟酚 e6 (Ce6) 集成到石墨烯量子点 (GQD) 或氧化石墨烯 (GO) 的平面结构上。即使在光照射条件下，所形成的杂化纳米体系也表现出相当大的荧光猝灭和轻微的光毒性，而PS的光活性在还原剂存在下可以选择性地恢复。与较大尺寸（约200 nm）的氧化石墨烯系统相比，GQD纳米系统通过增强的渗透和保留效应（EPR效应）表现出显着改善的肿瘤积累。体内研究表明，使用带有可裂解接头的 GQD 纳米系统治疗组可极其有效地抑制肿瘤生长，揭示了所提出的新策略的有前景的应用。
Prequenching and selective activation of photosensitizer (PS) are highly desired in photodynamic therapy (PDT) to avoid off target effect due to nonspecific activation and poor targeting selectivity of PS. In this study, nanographene materials as a unique x-conjugated planar System for electronic transfer were employed as the robust platform for temporarily quenching of PS. Photosensitizer chlorin e6 (Ce6) was integrated onto planar structure of graphene quantum dot (GQD) or graphene oxide (GO) via a reduction cleavable disulfide linker. The formed hybrid nanosystem displayed considerable fluorescence quenching and slight phototoxicity, even under the condition of light irradiation, while the photoactivity of PS could be selectively recovered in the presence of the reducing agent. Compared with graphene oxide system with larger size (around 200 nm), GQD nanosystern exhibited significantly improved tumor accumulation via enhanced permeation and retention;effect (EPR effect). The in vivo study demonstrated extremely effective suppression of tumor growth for the group treated with the GQD nanosystem with cleavable linker, revealing the promising application of the presented novel strategy.