Expression of intracellular calcium signalling genes in cattle skin during tick infestation
Expression of intracellular calcium signalling genes in cattle skin during tick infestation
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DOI：
10.1111/j.1365-3024.2008.01092.x
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发表时间：
2009-04-01
影响因子：
2.2
通讯作者：
Kongsuwan, K.
中科院分区：
文献类型：
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作者：
Bagnall, N.;Gough, J.;Kongsuwan, K.
细胞内钙离子（Ca2+）浓度的变化提供了控制大量细胞过程的动态信号，包括触发和介导宿主防御机制。本研究采用实时荧光定量PCR技术，分析了微小扇头蜱（Rhipicephalus（Boophilus）microplus）人工感染后高抗蜱（HR）和低抗蜱（LR）牛皮肤中14种Ca2+信号蛋白的基因表达。蜱攻击后，在HR和LR皮肤中均观察到许多基因的上调，但在HR组织中发现了显著更高的转录激活。HR皮肤中特异性Ca2+信号传导基因如AHNAK、CASQ、IL 2、NFAT 2CIP和PLCG 1的表达升高可能与宿主抗性有关。我们的数据表明，Ca2+及其相关蛋白可能在宿主对蜱的反应中发挥重要作用，并需要进一步研究。
It is widely acknowledged that changes in intracellular calcium ion (Ca2+) concentration provide dynamic signals that control a plethora of cellular processes, including triggering and mediating host defence mechanisms. In this study, quantitative real-time PCR was used to analyse gene expression of 14 Ca2+ signalling proteins in skin obtained from high tick-resistant (HR) and low tick-resistant (LR) cattle following artificial challenge with cattle tick (Rhipicephalus (Boophilus) microplus). Up-regulation of numerous genes was observed in both HR and LR skin following tick challenge, however substantially higher transcription activation was found in HR tissue. The elevated expression in HR skin of specific Ca2+ signalling genes such as AHNAK, CASQ, IL2, NFAT2CIP and PLCG1 may be related to host resistance. Our data suggest that Ca2+ and its associated proteins might play an important role in host response to ticks and that further investigation is warranted.