Bacteriocin diversity and the frequency of multiple bacterlocin production in Escherichia coli
Bacteriocin diversity and the frequency of multiple bacterlocin production in Escherichia coli
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DOI：
10.1099/mic.0.28690-0
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发表时间：
2006-11-01
期刊：
影响因子：
2.8
通讯作者：
O'Brien, Claire L.
中科院分区：
文献类型：
--
作者：
Gordon, David M.;O'Brien, Claire L.
本文收集了266株人类大肠杆菌粪便分离株，对其进行了产丝裂霉素c诱导菌素的检测，并采用基于pcr的方法筛选了11种粘连菌素和7种微粘连菌素。检测到8种不同的colicins和所有7种微cin。在被检测的菌株中，38%产生细菌素，24%产生大肠杆菌素，20%产生微霉素。在102种产生细菌素的菌株中，42%产生一种细菌素，41%产生两种细菌素，16%产生三种细菌素，发现一种菌株产生四种不同的细菌素。产生一种以上细菌素的菌株更有可能是大肠杆菌遗传群B2的成员，而不太可能属于遗传群A或d。研究发现，几种细菌素在一个菌株中同时出现的频率比偶然预期的要高：微霉素H47和M；粘菌素Ia和微霉素V；colicins B和M；colicins El和M；医生：我和我。由于细胞裂解而释放的细菌素没有被发现比偶然预期的更频繁地相互结合。提出了三个不相互排斥的假设，可以解释大肠杆菌菌株中多种细菌素的高频率产生：(1)扩大了杀灭范围，(2)扩大了受体库，(3)在不同环境中的适应性益处。
A collection of 266 faecal isolates of Escherichia coli from humans was assayed for the production of mitomycin C-inducible bacteriocins and screened using a PCR-based method for the presence of eleven colicins and seven microcins. Eight different colicins were detected and all seven microcins. Of the strains examined, 38 % produced a bacteriocin, 24 % produced a colicin and 20 % produced a microcin. Of the 102 bacteriocin-producing strains, 42 % produced one type of bacteriocin, 41 % produced two, 16 % produced three and one strain was found to produce four different bacteriocins. Strains producing more than one bacteriocin were more likely to be members of E coli genetic group B2 and less likely to belong to genetic groups A or D. Several of the bacteriocins were found to co-occur in a strain more often than would be expected by chance: microcins H47 and M; colicin Ia and microcin V; colicins B and M; colicins El and M; colicins Ell and Ia. No bacteriocins released as a consequence of cell lysis were found to co-associate more often than expected by chance. Three non-mutually exclusive hypotheses are presented that might explain the high frequency of multiple bacteriocin production in E coli strains: (1) expanded killing range, (2) expanded receptor repertoire and (3) fitness benefits in different environments.