The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.
The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.
复制标题
秀丽隐杆线虫中的 mRNA 输出由 Ce-NXF-1 介导，Ce-NXF-1 是人 TAP/NXF 和酿酒酵母 Mex67p 的直系同源物。
DOI：
10.1017/s1355838200000832
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Felber,BK
中科院分区：
文献类型：
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作者：
Tan,W;Zolotukhin,AS;Bear,J;Patenaude,DJ;Felber,BK
人TAP和酿酒酵母Mex 67 p属于介导mRNA输出的蛋白质家族。计算机搜索先前鉴定了两种秀丽隐杆线虫基因C15H11.3和C15H11.6，其编码hTAP和Mex 67 p的推定同源物（Segref等人，EMBO J，1997，16：3256-3271）。利用RNA干扰技术，在C.在线虫中，我们发现C15H11.3的功能性敲除导致含poly（A）的RNA在核中积累，并且对线虫的胚胎和成虫都是致命的。在C15H11.6的功能性敲除中未观察到胚胎或后代异常。综上所述，这些数据确定C15H11.3基因产物是hTAP和Mex 67 p的直系同源物;因此，将其命名为Ce-NXF-1。Ce-NXF-1直接结合RNA，是一种在核质和核边缘积累的核质穿梭蛋白。边缘结合通过所有TAP/NXF和Mex 67 p蛋白的C-末端部分中存在的独特信号介导。该区域显示与核孔蛋白Nup 98、Nup 153和Nup 214的FG重复结构域相互作用，表明边缘关联通过核孔复合物的组分发生。总之，Ce-NXF-1与hTAP和Mex 67 p一起属于参与mRNA输出的蛋白质家族，并且可以提供mRNA与核孔复合物组分之间的直接分子连接。因此，尽管这些物种之间的mRNA代谢存在差异，但它们利用保守的mRNA转运机制。
Human TAP and Saccharomyces cerevisiae Mex67p belong to a family of proteins that mediate mRNA export. Computer searches identified previously two Caenorhabditis elegans genes, C15H11.3 and C15H11.6, that encode putative homologs of hTAP and Mex67p (Segref et al., EMBO J, 1997, 16:3256–3271). Using RNA interference experiments in C. elegans, we found that functional knockout of C15H11.3 resulted in nuclear accumulation of poly(A)-containing RNAs and was lethal for both embryos and adult nematodes. No embryonic or progeny abnormality was observed in functional knockout of C15H11.6. Taken together, these data established that the C15H11.3 gene product is an ortholog of hTAP and Mex67p; thus, it was named Ce-NXF-1. Ce-NXF-1 binds RNA directly and is a nucleocytoplasmic shuttle protein accumulating in the nucleoplasm and at the nuclear rim. The rim association is mediated via unique signals present in the C-terminal portion of all TAP/NXF and Mex67p proteins. This region was shown to interact with the FG-repeat domains of nucleoporins Nup98, Nup153, and Nup214, indicating that the rim association occurs through components of the nuclear pore complex. In summary, Ce-NXF-1 belongs together with hTAP and Mex67p to a family of proteins that participate in mRNA export and can provide a direct molecular link between mRNAs and components of the nuclear pore complex. Therefore, despite differences in mRNA metabolism between these species, they utilize a conserved mRNA transport mechanism.