Depletion force induced collective motion of microtubules driven by kinesin
Depletion force induced collective motion of microtubules driven by kinesin
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DOI：
10.1039/c5nr02213d
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发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Kakugo, Akira
中科院分区：
文献类型：
--
作者：
Inoue, Daisuke;Mahmot, Bulbul;Kakugo, Akira
集体运动是自推进物体协调行为的一个有趣的例子，这通常与大规模模式的形成有关。目前，体外滑翔实验被认为是实验研究自行体群体行为和图案形成的各个方面的模型系统。在体外滑动实验中，细胞骨架细丝F-肌动蛋白或微管分别由表面固定的相关生物分子马达肌球蛋白或动力蛋白驱动。尽管F-肌动蛋白/肌球蛋白或微管/动力蛋白系统被发现在理解自推进物体的集体运动和图案形成方面很有前途，但最广泛使用的生物分子马达系统微管/动力蛋白在这方面至今还没有成功的应用。动蛋白驱动微管的集体运动失败归因于动蛋白的固有特性，该特性被认为影响了单个滑行微管的行为以及它们之间的相互作用。在这项工作中，我们首次通过调节滑动的微管之间的相互作用，通过在它们之间施加耗尽力来演示运动蛋白驱动的微管的集体运动。通过利用耗尽力适当地调节滑行微管之间的相互作用，使微管表现出集体运动和流型形成。这项工作为利用生物分子马达系统的体外滑动分析来演示集体运动提供了一种普遍的手段，并将有助于对自行物体迷人的协调行为和图案形成的细致理解。
Collective motion is a fascinating example of coordinated behavior of self-propelled objects, which is often associated with the formation of large scale patterns. Nowadays, the in vitro gliding assay is being considered a model system to experimentally investigate various aspects of group behavior and pattern formation by self-propelled objects. In the in vitro gliding assay, cytoskeletal filaments F-actin or microtubules are driven by the surface immobilized associated biomolecular motors myosin or dynein respectively. Although the F-actin/myosin or microtubule/dynein system was found to be promising in understanding the collective motion and pattern formation by self-propelled objects, the most widely used biomolecular motor system microtubule/kinesin could not be successfully employed so far in this regard. Failure in exhibiting collective motion by kinesin driven microtubules is attributed to the intrinsic properties of kinesin, which was speculated to affect the behavior of individual gliding microtubules and mutual interactions among them. In this work, for the first time, we have demonstrated the collective motion of kinesin driven microtubules by regulating the mutual interaction among the gliding microtubules, by employing a depletion force among them. Proper regulation of the mutual interaction among the gliding microtubules through the employment of the depletion force was found to allow the exhibition of collective motion and stream pattern formation by the microtubules. This work offers a universal means for demonstrating the collective motion using the in vitro gliding assay of biomolecular motor systems and will help obtain a meticulous understanding of the fascinating coordinated behavior and pattern formation by self-propelled objects.