NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
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DOI：
10.1111/pce.12711
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发表时间：
2017-04-01
影响因子：
7.3
通讯作者：
Sandalio, L. M.
中科院分区：
文献类型：
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作者：
Gupta, D. K.;Pena, L. B.;Sandalio, L. M.
以拟南芥（Arabidopsis thaliana）突变体AtrbohC、AtrbohD和AtrbohF为材料，在25和100 M镉溶液中培养1天和5d，研究了镉（Cd）胁迫下NADPH氧化酶的作用。镉抑制了WT、AtrbohC和D的叶片生长，但对AtrbohF没有影响。一个时间依赖性增加H2 O2和脂质过氧化反应，观察在所有的基因型，与AtrbohC表现出最小的增幅。相反的行为，观察到NO的积累。镉增加过氧化氢酶活性在野生型植物和减少它在Atrboh突变体，而谷胱甘肽还原酶和乙醇酸氧化酶活性增加在Atrboh突变体，超氧化物歧化酶下调在AtrbohC。GSH/GSSG和阿萨/DHA对也受到治疗的影响，主要分别在AtrbohC和AtrbohF中。处理1d后，AtrbohF突变体叶片中的镉转运量严重减少，甚至在处理5d后AtrbohF突变体叶片中的镉转运量也严重减少。S、P、Ca、Zn和Fe的积累也有类似的结果，而K的积累则有相反的趋势，AtrbohF除外。在镉胁迫下，呼吸爆发氧化酶（RBOH）同源物对H2 O2的产生有不同的调节作用，其中RBOHC是活性氧的最重要来源;抗氧化防御也受到不同的调节，RBOHC对超氧化物歧化酶的调节，RBOHC和D对氧化还原偶GSH/GSSG比率的调节以及RBOHF对阿萨/DHA的调节是最重要的因素。我们的研究结果还表明，RBOH可以发挥重要作用，在调节根到地上部的营养物质运输和营养调控中心的重要球员，例如，可以在生物技术的潜在利益，以保护重金属的拍摄。
The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohF, which were grown under hydroponic conditions with 25 and 100M Cd for 1 and 5days. Cadmium reduced the growth of leaves in WT, AtrbohC and D, but not in AtrbohF. A time-dependent increase in H2O2 and lipid peroxidation was observed in all genotypes, with AtrbohC showing the smallest increase. An opposite behaviour was observed with NO accumulation. Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated in AtrbohC. The GSH/GSSG and ASA/DHA couples were also affected by the treatment, principally in AtrbohC and AtrbohF, respectively. Cadmium translocation to the leaves was severely reduced in Atrboh mutants after 1day of treatment and even after 5days in AtrbohF. Similar results were observed for S, P, Ca, Zn and Fe accumulation, while an opposite trend was observed for K accumulation, except in AtrbohF. Thus, under Cd stress, RBOHs differentially regulate ROS metabolism, redox homeostasis and nutrient balance and could be of potential interest in biotechnology for the phytoremediation of polluted soils.Under cadmium stress, respiratory burst oxydase homologs (RBOHs) differentially regulate H2O2 production, with RBOHC being the most important source of reactive oxygen species; anti-oxidative defences are also differentially regulated, with superoxide dismutase regulation by RBOHC, as well as the regulation of redox-couple GSH/GSSG ratio by RBOHC and D and the ASA/DHA by RBOHF being the most important factors. Our results also suggest that RBOHs can play an important role in regulating the root-to-shoot nutrient transport and are important players in the nutrient regulatory hub and, for instance, can be of potential interest in biotechnology to protect shoot from heavy metals.