Enhanced Hyperthermic Responses to Lipopolysaccharide in Mice Exposed to Repeated Cold Stress.
Enhanced Hyperthermic Responses to Lipopolysaccharide in Mice Exposed to Repeated Cold Stress.
复制标题
暴露于反复冷应激的小鼠对脂多糖的高温反应增强。
DOI：
10.1159/000454815
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kawabata A.
中科院分区：
文献类型：
--
作者：
Miyamoto T1;Funakami Y;Kawashita E;Tomita S;Nomura A;Sugimoto N;Saeki H;Miyazakia T;Tsubota M;Ichida S;Kawabata A.
脂多糖（LPS）诱导高热伴随着各种其他全身性炎症症状。根据特定的时间表暴露于重复冷（RC）应激的啮齿动物可用作自主神经失衡或纤维肌痛的实验模型。现已证明RC应激小鼠对LPS表现出耐受性，我们通过使用遥测系统监测核心温度来检测RC应激小鼠对LPS攻击的热反应。全身给药LPS引起RC应激和非应激小鼠的双峰高热反应。LPS诱导的体温升高的幅度在RC应激小鼠中比在非应激小鼠中更大。用双氯芬酸（一种环氧合酶（考克斯）抑制剂）预处理可消除RC应激诱导的对LPS的热反应增强。脂多糖并没有显着增加考克斯-2蛋白水平在肺或下丘脑的RC应激或非应激小鼠。RC应激并没有改变基线血清皮质酮水平或其增加响应LPS的挑战。这些结果表明，RC应激增强了小鼠对LPS攻击的易感性，导致更大的前列腺素依赖性高热，这可能有助于RC应激小鼠对LPS的耐受性。
Lipopolysaccharide (LPS) induces hyperthermia accompanied by various other systemic inflammatory symptoms. The rodents exposed to repeated cold (RC) stress according to a specific schedule are useful as experimental models for autonomic imbalance or fibromyalgia. It is now proven that RC-stressed mice exhibit tolerance to LPS, we examined thermal responses to LPS challenge in RC-stressed mice by monitoring core temperature using the telemetry system. Systemic administration of LPS caused bimodal hyperthermic responses in RC-stressed and unstressed mice. The magnitude of the LPS-induced hyperthermia was greater in RC-stressed mice than in unstressed mice. The RC stress-induced enhancement of hyperthermic responses to LPS was abolished by pretreatment with diclofenac, which is a cyclooxygenase (COX) inhibitor. LPS did not significantly increase COX-2 protein levels in the lung or hypothalamus of RC-stressed or unstressed mice. RC stress did not alter baseline serum corticosterone levels or their increases in response to LPS challenge. These results suggest that RC stress enhances the susceptibility of mice to LPS challenge, leading to greater prostanoid-dependent hyperthermia, which might contribute to tolerance to LPS in RC-stressed mice.