NADPH-DIAPHORASE - A SELECTIVE HISTOCHEMICAL MARKER FOR THE CHOLINERGIC NEURONS OF THE PONTINE RETICULAR-FORMATION
NADPH-DIAPHORASE - A SELECTIVE HISTOCHEMICAL MARKER FOR THE CHOLINERGIC NEURONS OF THE PONTINE RETICULAR-FORMATION
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DOI：
10.1016/0304-3940(83)90124-6
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
FIBIGER, HC
中科院分区：
文献类型：
--
作者：
VINCENT, SR;SATOH, K;FIBIGER, HC
胆碱乙酰转移酶免疫组织化学已经确定了一个大组的胆碱能神经元在大鼠脑桥被盖。结合免疫组织化学和酶组织化学研究表明，这个特殊的胆碱能细胞群含有一种酶，NADPH-黄递酶，可以可视化组织化学。NADPH-黄递酶组织化学法是一种简便、可靠的选择性染色脑干网状结构胆碱能神经元的方法。通过这种新的组织化学技术获得的分辨率是类似的发现与高尔基染色，并应在此胆碱能细胞群的形态学研究的价值很大。
Choline acetyltransferase immunohistochemistry has identified a large group of cholinergic neurons in the rat pontine tegmentum. Combined immunohistochemical and enzyme histochemical studies revealed that this particular cholinergic cell group contains an enzyme, NADPH-diaphorase, that can be visualized histochemically. NADPH-diaphorase histochemistry provides a simple, reliable method to selectively stain the cholinergic neurons of the brainstem reticular formation. The resolution obtained by this novel histochemical technique is similar to that found with the Golgi stain, and should be of great value in morphological studies of this cholinergic cell group.