RFLP analysis of the PCR-amplified 28S rDNA inRhizoctonia solani
RFLP analysis of the PCR-amplified 28S rDNA inRhizoctonia solani
复制标题
立枯丝核菌 PCR 扩增 28S rDNA 的 RFLP 分析
DOI：
10.1007/bf02461308
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发表时间：
1996
期刊：
影响因子：
1.4
通讯作者：
N. Matsuyama
中科院分区：
文献类型：
--
作者：
M. Matsumoto;N. Furuya;Y. Takanami;N. Matsuyama
通过使用四种限制性内切核酸酶对代表立枯丝核菌的7个吻合组(AG)的13个种内组(ISG)的57个分离株进行28S rDNA基因区的一部分的RFLP分析。在三个AG（AG 1、2和4）中观察到PCR扩增的rDNA产物和限制性内切酶（BamHI、HaeIII、HhaI和HpaII）消化的多态性的变化。这些差异在AG 1和AG 2的一些ISG中也保守。在AG 1的ISG中，用HpaII消化获得的AG 1-IA的rDNA片段模式与AG显着不同。 1-IB和IC。通过用HhaI和HpaII消化，在AG 2-1、2-2 IIIB和2-2 IV中也观察到片段模式的这种差异。从限制酶数据得出的树状图显示，来自 AG 1 和 AG 2 的 ISG 可以各自细分为不同的组，这些组与其他 AG 的大多数分离株有较远的相关性。
RFLP analyses of a portion of the 28S rDNA gene region were conducted by using four restriction endonucleases for 57 isolates of 13 intraspecific groups (ISGs) representing 7 anastomosis groups (AGs) ofRhizoctonia solani. Variations in the PCR-amplified rDNA products and the polymorphisms on digestion with restriction enzymes (BamHI,HaeIII,HhaI andHpaII) were observed among three AGs, AG 1, 2 and 4. These differences were also conserved among some ISGs of AG 1 and AG 2. Among ISGs of AG 1, the pattern of rDNA fragments of AG 1-IA obtained by digestion withHpaII was significantly different from those of AG 1-IB and IC. Such difference in the fragment pattern was also observed among AG 2-1, 2-2 IIIB and 2-2 IV by the digestion withHhaI andHpaII. A dendrogram derived from the restriction enzyme data showed that ISGs from AG 1 and AG 2 can each be subdivided into distinct groups, those are distantly related to the majority isolates of the other AGs.