Nucleotide Sequence of a Soybean Vegetative Storage Protein vspA Gene.
Nucleotide Sequence of a Soybean Vegetative Storage Protein vspA Gene.
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大豆营养储存蛋白 vspA 基因的核苷酸序列。
DOI：
10.1104/pp.98.2.792
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发表时间：
1992
期刊：
影响因子：
7.4
通讯作者：
P. Staswick
中科院分区：
文献类型：
--
作者：
Yoon Rhee;P. Staswick
大豆（甘氨酸max） [L]；)稳定)。VSP2由两个相似的多肽组成，分别命名为VSP-a和VSP- 3，它们来源于相应的基因vspA和vspB。这些基因引起了相当大的兴趣，因为它们受几种发育和环境因素以及茉莉酸（一种新发现的植物生长调节剂）的调节(5)。来自两个VSP基因的cdna序列已经公布（2,4），vspB的基因组序列也已公布(3)。我们对这些基因进行了克隆和测序，以研究其启动子在基因调控中的功能。本文报道的vspA基因序列此前未见报道。我们克隆了一个8.5 kb的xbai - ecorigomic片段，该片段与vspA cDNA探针特异性杂交。对该片段的两条亚克隆链进行测序，跨越3.3 kb的区域（表1，图1）。这包括转录起始位点上游1.5 kb，这是由引物延伸确定的。编码序列与先前公布的cDNA序列一致（2,4）。一个假定的塔塔盒子位于32号（塔塔）。我们注意到，位于at- 18的短序列（AGTAAA）存在于VSP基因和其他几个伤口诱导基因中（PE Staswick，未发表的结果），包括马铃薯蛋白酶抑制剂II，它也可由茉莉酸甲酯诱导(1)。
Soybean (Glycine max [L.] Merr.) VSP2 consists of two similar polypeptides, designated VSP-a and VSP-, 3, that are derived from the corresponding genes, vspA and vspB. These genes are ofconsiderable interest because they are modulated by several developmental and environmental factors and by jasmonic acid, a newlyrecognized putative plant growth regulator (5). The sequence ofcDNAs derived from both VSP genes has been published (2, 4), as has a genomic sequence for vspB (3). We have cloned and sequenced these genes to study the function of their promoters in gene regulation. The vspA gene sequence presented here has not been reported previously.We cloned an 8.5-kb XbaI-EcoRIgenomic fragment that hybridized specifically to a vspA cDNA probe. Both strands of subclones from this fragment were sequenced, spanning a region of 3.3 kb (Table I, Fig. 1). This included 1.5 kb upstream ofthe transcription start site, which was determined by primer extension. The coding sequence was identical with the cDNA sequence published previously (2, 4). A putative TATA box is located at-32 (TATTTA). We noted that a short sequence located at-1 18 (AGTAAA) was present in both VSP genes and in several other wound-inducible genes (PE Staswick, unpublished results), including potato pro-teinase inhibitor II which is also inducible by methyl jasmon-ate (1).