SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS
SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS
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DOI：
10.1016/0003-2697(71)90370-8
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发表时间：
1971-01-01
影响因子：
2.9
通讯作者：
FRIDOVIC.I
中科院分区：
文献类型：
--
作者：
BEAUCHAM.C;FRIDOVIC.I
硝基蓝四氮唑已被用来拦截酶或光化学产生的O2−。超氧化物歧化酶被O2-−还原为超氧化物歧化酶的基础，通过抑制还原反应暴露了超氧化物歧化酶的存在。因此，可以在粗提物或纯化的蛋白质组分中检测超氧化物歧化酶。本方法对超氧化物歧化酶的浓度为ng/ml敏感，适用于无水溶液或聚丙烯酰胺凝胶。在聚丙烯酰胺电泳图上定位超氧化物歧化酶的染色程序已被应用于从各种来源获得的提取物。已发现大肠杆菌含有两种超氧化物歧化酶，而牛心、脑、肺和红细胞只含有一种。
Nitro blue tetrazolium has been used to intercept O2−generated enzymically or photochemically. The reduction of NBT by O2−has been utilized as the basis of assays for superoxide dismutase, which exposes its presence by inhibiting the reduction of NBT. Superoxide dismutase could thus be assayed either in crude extracts or in purified protein fractions. The assays described are sensitive to ng/ml levels of super-oxide dismutase and were applicable in free solution or on polyacrylamide gels. The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources. E. coli has been found to contain two superoxide dismutases whereas bovine heart, brain, lung, and erthrocytes contain only one.