On-Chip Parylene-C Microstencil for Simple-to-Use Patterning of Proteins and Cells on Polydimethylsiloxane
On-Chip Parylene-C Microstencil for Simple-to-Use Patterning of Proteins and Cells on Polydimethylsiloxane
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DOI：
10.1021/am4001166
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发表时间：
2013-04-10
影响因子：
9.5
通讯作者：
Yang, Sung
中科院分区：
文献类型：
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作者：
Lee, Donghee;Yang, Sung
聚二甲基硅氧烷（PDMS）被广泛用作小型设备的底物，因为它适合执行生物和化学分析。在这里，我们提出了一种PDMS的模式方法，它使用芯片上的聚苯乙烯-c微模板来模式蛋白质和细胞。为了实现片上的聚苯乙烯-c微模板，我们使用正硅酸四乙酯（TEOS）与氧气混合的常压等离子体增强化学气相沉积（AP-PECVD）应用了siox类纳米颗粒层。通过各种表面表征分析，包括光学透明度、表面形貌、化学成分和剥离力，证明了在应用siox类纳米颗粒层后，PDMS中二甲苯- c的完全去除。此外，还研究了AP-PECVD处理次数的影响。我们的方法克服了聚苯乙烯- c从PDMS上不完全脱落的趋势，这限制了它与PDMS的使用。基于PDMS上的这种聚苯乙烯-c剥离过程的片上聚苯乙烯-c微模板方法可以在2 μ m分辨率下对蛋白质和单细胞分辨率下的细胞进行建模，空位率低至10%。这提供了优越的用户友好性和更大程度的几何自由度，而不是以前描述的方法，需要在处理模板时小心翼翼。因此，该方法可以应用于各种研究领域，在柔性PDMS底物上对蛋白质或细胞进行图案化。
Polydimethylsiloxane (PDMS) is widely used as a substrate in miniaturized devices, given its suitability for execution of biological and chemical assays. Here, we present a patterning approach for PDMS, which uses an on-chip Parylene-C microstencil to pattern proteins and cells. To implement the on-chip Parylene-C microstencil, we applied SiOx-like nanoparticle layers using atmospheric-pressure plasma-enhanced chemical vapor deposition (AP-PECVD) of tetraethyl orthosilicate (TEOS) mixed with oxygen. The complete removal of Parylene-C from PDMS following application of SiOx-like nanoparticle layers was demonstrated by various surface characterization analysis, including optical transparency, surface morphology, chemical composition, and peel-off force. Furthermore, the effects of the number of AP-PECVD treatments were investigated. Our approach overcomes the tendency of Parylene-C to peel off incompletely from PDMS, which has limited its use with PDMS to date. The on-chip Parylene-C microstencil approach that is based on this Parylene-C peel-off process on PDMS can pattern proteins with 2-mu m resolution and cells at single-cell resolution with a vacancy ratio as small as 10%. This provides superior user-friendliness and a greater degree of geometrical freedom than previously described approaches that require meticulous care in handling of stencil. Thus, this patterning method could be applied in various research fields to pattern proteins or cells on the flexible PDMS substrate.