Hydroxyapatite-coated double network hydrogel directly bondable to the bone: Biological and biomechanical evaluations of the bonding property in an osteochondral defect
Hydroxyapatite-coated double network hydrogel directly bondable to the bone: Biological and biomechanical evaluations of the bonding property in an osteochondral defect
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DOI：
10.1016/j.actbio.2016.08.016
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发表时间：
2016-10-15
影响因子：
9.7
通讯作者：
Yasuda, Kazunori
中科院分区：
文献类型：
--
作者：
Wada, Susumu;Kitamura, Nobuto;Yasuda, Kazunori
我们开发了一种新型的羟基磷灰石（HAp）涂层双网络（DN）水凝胶（HAp/DN凝胶）。本研究的目的是确定植入HAp/DN凝胶周围的细胞和组织反应的细节，并确定在兔骨软骨缺损模型中HAp/DN凝胶与骨结合的速度和强度。2周时在HAp/DN凝胶与骨之间形成未成熟的类骨质组织，4周时类骨质组织矿化。HAp/DN凝胶在4周和12周时的平均推出载荷分别为37.54 N和42.15 N，而DN凝胶的平均推出载荷小于5 N。HAp/DN凝胶与骨的结合面积在4周时大于80%，在12周时大于90%。这项研究表明，HAp/DN凝胶在植入后的早期阶段增强了骨整合。纳米结构的存在下，除了骨整合的HAp促进成骨细胞粘附到表面的HAp/DN凝胶。HAp/DN凝胶具有改善下一代骨科植入物（如人工软骨）中植入物-组织界面的潜力。重要性声明最近的研究报告了各种水凝胶的发展，这些水凝胶具有足够的韧性，可用作软支持组织。然而，以适当的强度将水凝胶固定在骨表面上是一个巨大的挑战。我们已经开发了一种新的，坚韧的水凝胶杂交羟基磷灰石（HAp/DN凝胶），这是直接结合到骨。目前的研究表明，HAp/DN凝胶在植入后的早期阶段增强了骨整合。除了羟基磷灰石的骨整合能力之外，纳米结构的存在促进了成骨细胞粘附到HAp/DN凝胶的表面上。HAp/DN凝胶具有改善下一代骨科植入物（如人工软骨）中植入物-组织界面的潜力。(C)2016 Acta Materialia Inc.由爱思唯尔有限公司发布。保留所有权利。
We have developed a novel hydroxyapatite (HAp)-coated double-network (DN) hydrogel (HAp/DN gel). The purpose of this study was to determine details of the cell and tissue responses around the implanted HAp/DN gel and to determine how quickly and strongly the HAp/DN gel bonds to the bone in a rabbit osteochondral defect model. Immature osteoid tissue was formed in the space between the HAp/DN gel and the bone at 2 weeks, and the osteoid tissue was mineralized at 4 weeks. The push-out load of the HAp/DN gel averaged 37.54 N and 42.15 N at 4 and 12 weeks, respectively, while the push-out load of the DN gel averaged less than 5 N. The bonding area of the HAp/DN gel to the bone was above 80% by 4 weeks, and above 90% at 12 weeks. This study demonstrated that the HAp/DN gel enhanced osseointegration at an early stage after implantation. The presence of nanoscale structures in addition to osseointegration of HAp promoted osteoblast adhesion onto the surface of the HAp/DN gel. The HAp/DN gel has the potential to improve the implant-tissue interface in next-generation orthopaedic implants such as artificial cartilage.Statement of SignificanceRecent studies have reported the development of various hydrogels that are sufficiently tough for application as soft supporting tissues. However, fixation of hydrogels on bone surfaces with appropriate strength is a great challenge. We have developed a novel, tough hydrogel hybridizing hydroxyapatite (HAp/DN gel), which is directly bondable to the bone. The present study demonstrated that the HAp/DN gel enhanced osseointegration in the early stage after implantation. The presence of nanoscale structures in addition to the osseointegration ability of hydroxyapatite promoted osteoblast adhesion onto the surface of the HAp/DN gel. The HAp/DN gel has the potential to improve the implant-tissue interface in next-generation orthopaedic implants such as artificial cartilage. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.