Genome-wide identification of lipases in silkworm (Bombyx mori) and their spatio-temporal expression in larval midgut.
Genome-wide identification of lipases in silkworm (Bombyx mori) and their spatio-temporal expression in larval midgut.
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DOI：
10.1016/j.gene.2021.146121
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发表时间：
2021-12
期刊：
影响因子：
3.5
通讯作者：
Yunwang Shen;Guanping Chen;Shudi Zhao;Xiaofeng Wu
中科院分区：
文献类型：
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作者：
Yunwang Shen;Guanping Chen;Shudi Zhao;Xiaofeng Wu
脂肪酶通过将膳食脂质降解为游离脂肪酸和甘油，在食物消化中发挥至关重要的作用。几十年来，家蚕（Bombyx mori）已被广泛用作重要的鳞翅目模型。然而，人们对蚕的脂肪酶基因家族知之甚少，特别是它们作为消化酶的水解活性。本研究在家蚕基因组中共鉴定出 38 个脂肪酶基因。系统发育分析表明它们分为三个主要类群。十二种脂肪酶被证实在中肠中在转录和翻译水平上表达。它们被归入相同的基因簇，表明它们可能具有相似的生理功能。实时定量PCR（qRT-PCR）分析表明，脂肪酶主要在中肠前区和中肠区表达，且其表达水平沿中肠长度变化很大。当幼虫停止进食时，中肠中的大多数脂肪酶都会下调。然而，一个独特的脂肪酶基因（Bmlip10583）在摄食阶段表现出低表达水平，但在幼虫-蛹转变过程中显着上调。这些结果表明，在幼虫发育过程中，蚕脂肪酶的表达在中肠中受到空间和时间上的调节。总而言之，我们的结果为蚕脂肪酶基因家族提供了基础研究。
Lipases play crucial roles in food digestion by degrading dietary lipids into free fatty acids and glycerols. The domesticated silkworm (Bombyx mori) has been widely used as an important Lepidopteran model for decades. However, little is known about the lipase gene family in the silkworm, especially their hydrolytic activities as digestive enzymes. In this study, a total of 38 lipase genes were identified in the silkworm genome. Phylogenetic analysis indicated that they were divided into three major groups. Twelve lipases were confirmed to be expressed in the midgut at both transcriptional and translational levels. They were grouped into the same gene cluster, suggesting that they could have similar physiological functions. Quantitative real-time PCR (qRT-PCR) analyses indicated that lipases were mainly expressed in anterior and middle midgut regions, and their expression levels varied greatly along the length of midgut. A majority of lipases were down-regulated in the midgut when larvae stopped feeding. However, a unique lipase gene (Bmlip10583) showed low expression level during feeding stage, but it was significantly up-regulated during the larvae-pupae transition. These results demonstrated that expression of silkworm lipases was spatially and temporally regulated in the midgut during larval development. Taken together, our results provide a fundamental research of the lipase gene family in the silkworm.