STUDIES ON NONCOOPERATIVE BINDING OF ESCHERICHIA-COLI DNA UNWINDING PROTEIN TO SINGLE-STRANDED NUCLEIC-ACIDS
STUDIES ON NONCOOPERATIVE BINDING OF ESCHERICHIA-COLI DNA UNWINDING PROTEIN TO SINGLE-STRANDED NUCLEIC-ACIDS
复制标题
DOI：
10.1021/bi00668a017
复制
发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
WETMUR, JG
中科院分区：
文献类型：
--
作者：
RUYECHAN, WT;WETMUR, JG
E.用平衡透析法研究了大肠杆菌DNA解旋蛋白对单链DNA寡聚体的影响。在许多溶液和温度条件下使用不同长度和碱组成的低聚物进行透析。从Scatchard分析的结合和其他结果，提出了一个模型的蛋白质的单链DNA的合作结合。还介绍了蛋白质与单链RNA的相互作用。
The noncooperative binding of the E. coli DNA unwinding protein to single-stranded DNA oligomers was studied by equilibrium dialysis. Dialyses were performed under a number of solution and temperature conditions using oligomers of varying length and base composition. From Scatchard analysis of the binding and other results, a model for the cooperative binding of the proteins to single-stranded DNA is proposed. The interaction of the protein with single-stranded RNA is also presented.