L-lactate dehydrogenase and N-acetyl-β-D-glucosaminidase activities in bovine milk as indicators of non-specific mastitis
L-lactate dehydrogenase and N-acetyl-β-D-glucosaminidase activities in bovine milk as indicators of non-specific mastitis
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DOI：
10.1017/s0022029906001956
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发表时间：
2006-11-01
影响因子：
2.1
通讯作者：
Ingvartsen, Klaus L.
中科院分区：
文献类型：
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作者：
Chagunda, Mizeck G. G.;Larsen, Torben;Ingvartsen, Klaus L.
研究了影响L-乳酸脱氢酶（LDH）和N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）活性和体细胞计数（SCC）的系统性因素，LDH和NAGase活性与SCC与乳房健康状况之间的相关性，以及LDH和NAGase将奶牛分为乳房健康类别以早期检测乳腺炎的能力。一个数据集的记录，从74丹麦荷斯坦，76丹麦红和47泽西奶牛在一个研究农场。将奶牛分为健康奶牛和临床乳腺炎奶牛。健康奶牛定义为未经兽医治疗且SCC < 100 000个细胞/ml。临床感染的奶牛是在显示乳腺炎和SCC > 800000个细胞/ml的临床体征后接受兽医治疗的奶牛。品种、产奶月份和产奶天数对健康和临床乳腺炎奶牛的LDH活性、NAGase活性和SCC均有显著影响（P < 0.001）。在健康奶牛中，LDH活性、NAGase活性和SCC在产犊后立即开始处于高水平，并在产后约30-40 d降至低水平。由于临床型乳腺炎，所有三个参数均增加。NAGase活性在健康奶牛中的变异性在数值上高于临床乳腺炎奶牛（CV = 56 - 2% v. CV = 53.5%）。LDH活性和SCC之间的关系是更强的临床乳腺炎比从健康奶牛的牛奶（r = 0.76 v. r = 0.48和r = 0.67 v. r = 0.44的观察值和残差的相关性，分别）。LDH活性比NAGase活性具有更高的灵敏度（73-95%对35-77%），而特异性在相似的范围内（92-99%）。此外，LDH活性的灵敏度对阈值的变化比NAGase活性的灵敏度更稳健。自动化，在线实时乳腺炎检测的机会进行了讨论。
Systematic factors affecting the activities of L-lactate dehydrogenase (LDH) and N-acetyl-beta-D-glucosaminidase (NAGase) and somatic cell count (SCC), the association between the activities of LDH and NAGase and SCC with respect to udder health status, and the ability of LDH and NAGase to classify cows in udder health categories for early detection of mastitis were studied. A dataset of records from 74 Danish Holstein, 76 Danish Red and 47 Jersey cows on one research farm was used. Cows were grouped into healthy and clinically mastitic. A healthy cow was defined as having no veterinary treatment and SCC < 100 000 cells/ml. A clinically infected cow was one receiving veterinary treatment after showing clinical signs of mastitis and SCC > 800000 cells/ml. Breed, month of production, and days in milk significantly influenced (P < 0.001) LDH activity, NAGase activity and SCC in both healthy and clinically mastitic cows. In healthy cows, LDH activity, NAGase activity and SCC started at a high level immediately after calving and decreased to low levels approximately 30-40 d post partum. All the three parameters increased due to clinical mastitis. NAGase activity had numerically higher variation in healthy cows than in clinically mastitic cows (CV = 56 - 2% v. CV = 53.5%). The relationship between LDH activity and SCC was stronger in milk from clinically mastitic than from healthy cows (r = 0.76 v. r = 0.48 and r = 0.67 v. r = 0.44 for correlation of observed values and residuals, respectively). LDH activity had higher sensitivity than NAGase activity (73-95% v. 35-77%) while specificities were in a similar range (92-99%). Further, sensitivities for LDH activity were more robust to changes in the threshold value than those for NAGase activity. Opportunities for automated, in-line real-time mastitis detection are discussed.