Insertion mechanism of cell-penetrating peptides into supported phospholipid membranes revealed by X-ray and neutron reflection
Insertion mechanism of cell-penetrating peptides into supported phospholipid membranes revealed by X-ray and neutron reflection
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DOI：
10.1039/c2sm25913c
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Shin, K.
中科院分区：
文献类型：
--
作者：
Choi, D.;Moon, J. H.;Shin, K.
由1,2-双棕榈酰- n-甘油-3-磷脂胆碱（DPPC）双分子层和转录激活因子衍生肽（TDPs）组成的系统的x射线和中子反射率测量使我们能够确定膜易位的机制。通过监测系统改变温度和TDP浓度时TDP结合引起的双分子层结构变化，我们的研究结果揭示了TDP在脂质双分子层易位过程中发生的逐步相互作用的详细分子结构。虽然在低TDP浓度下几乎没有观察到膜扰动的迹象，但我们发现在较高TDP浓度下，TDP在膜上的运动诱导了膜上的缺陷形成。
X-Ray and neutron reflectivity measurements on systems composed of a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayer and transcription-activating-factor derived peptides (TDPs) have allowed us to determine the mechanism of membrane translocation. By monitoring the structural changes of the bilayers caused by the binding of TDPs while systemically varying temperature and TDP concentration, our results revealed the detailed molecular structures of the stepwise interactions that occurred during the translocation of TDP across the lipid bilayers. While little indication of membrane perturbation was observed at low TDP concentrations, we found that the TDP movement across the membrane induced defect formations in the membrane at higher TDP concentrations.