Immunologic response to Bartonella henselae as determined by enzyme immunoassay and Western blot analysis.
Immunologic response to Bartonella henselae as determined by enzyme immunoassay and Western blot analysis.
复制标题
通过酶免疫测定和蛋白质印迹分析确定对汉赛巴尔通体的免疫反应。
DOI：
10.1093/ajcp/108.2.202
复制
发表时间：
1997
影响因子：
3.5
通讯作者：
Hill,HR
中科院分区：
文献类型：
--
作者：
Litwin,CM;Martins,TB;Hill,HR
Bartonella henselaeis now regarded as the etiologic agent of catscratch disease and a cause of bacillary angiomatosis. We examined the human immune response toBartonella henselaeinfection using a newly developed enzyme immunoassay (EIA) and a Western blot procedure using outer-membrane proteins. The EIA showed 98.6% and 91.4% agreement with an indirect fluorescence method (IFA) for detection of IgM and IgG antibodies, respectively. By using Western blot analysis, reactivity to an 8-kd band showed significant correlation with positive results by the IgM IFA and EIA. In contrast, reactivity to 209-, 208.5-, 208-, 116-, and 80-kd bands was identified only in positive IgG IFA serum samples. The EIA and Western blot should be useful tests in determining the antibody response toB henselaeinfection and may also be important in determining the critical epitopes in the hostparasite interaction of this organism.
