The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity
The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity
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DOI：
10.1016/j.virol.2010.03.047
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发表时间：
2010-07-20
期刊：
影响因子：
3.7
通讯作者：
de Haan, Cornelis A. M.
中科院分区：
文献类型：
--
作者：
de Vries, Robert P.;de Vries, Erik;de Haan, Cornelis A. M.
在这项研究中，我们评估了重组可溶性血凝素（HA）三聚体（亚型H2和H7）在昆虫S2细胞，人HEK 293 T或HEK 293 S GnTI（-）细胞中产生的受体结合特性，这些细胞分别产生少甘露糖，复合物或高甘露糖N-连接聚糖的蛋白质。结果表明，HA蛋白，只有不同的糖基化状态具有不同的受体精细的特异性。HEK 293 T细胞产生的HA显示出非常窄的受体特异性。然而，当用神经氨酸酶处理时，该HA能够以与HEK 293 S GnTI（-）细胞产生的HA相似的特异性结合更多的聚糖。昆虫细胞产生的HA表现出受体特异性降低。因此，在昆虫细胞中无法观察到HA精细受体特异性的差异，但在HEK 293 S GnTI（-）细胞产生的HA中很容易检测到。(C)2010年爱思唯尔公司All rights reserved.
In this study we evaluated the receptor-binding properties of recombinant soluble hemagglutinin (HA) trimers (subtype H2 and H7) produced in insect S2 cells, human HEK293T or HEK293S GnTI(-) cells, which produce proteins with paucimannose, complex or high-mannose N-linked glycans, respectively. The results show that HA proteins that only differ in their glycosylation status possess different receptor fine specificities. HEK293T cell-produced HA displayed a very narrow receptor specificity. However, when treated with neuraminidase this HA was able to bind more glycans with similar specificity as HEK293S GnTI(-) cell-produced HA. Insect cell-produced HA demonstrated decreased receptor specificity. As a consequence, differences in HA fine receptor specificities could not be observed with the insect cell-, but were readily detected with the HEK293S GnTI(-) cell-produced HAs. (C) 2010 Elsevier Inc. All rights reserved.