Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration
Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration
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人羊膜上皮细胞的成骨分化及其在肺泡缺损修复中的应用
DOI：
10.5966/sctm.2014-0118
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发表时间：
2014-12-01
影响因子：
6
通讯作者：
Guo Lihe
中科院分区：
文献类型：
--
作者：
Si Jiawen;Zhang Jianjun;Guo Lihe
本研究对人羊膜上皮细胞（human amniotic epithelial cells，hAECs）的体外成骨分化过程和体内骨再生特性进行了详细的研究。通过生化染色、实时荧光定量聚合酶链反应、免疫荧光等方法观察hAECs体外成骨分化过程。接下来，将单独的β-磷酸三钙（β-TCP）支架或装载有hAEC的支架植入大鼠的肺泡缺损中。进行了显微计算机断层扫描评价和组织学研究。我们的研究结果通过Runx 2、osterix、碱性磷酸酶、I型胶原和骨桥蛋白的上调证实了hAEC的体外成骨能力，成骨细胞的生化染色阳性。上皮-间充质转化过程可能通过增加转化生长因子β 1的表达而参与hAEC的成骨分化。我们的数据还表明，在体内植入的hArCs负载在β-TCP支架，不仅提高了骨再生的直接参与，但也减少了早期宿主免疫反应的支架。所提供的数据表明，hAEC具有适当的成骨分化潜力和对早期组织重塑过程的调节作用，使这些细胞成为临床修复牙槽骨缺损的祖细胞的潜在来源。
The present study investigated the detailed in vitro osteogenic differentiation process and in vivo bone regenerative property of human amniotic epithelial cells (hAECs). The in vitro osteogenic differentiation process of hAECs was evaluated by biochemical staining, real-time polymerase chain reaction, and immunofluorescence. Next, beta-tricalcium phosphate (beta-TCP) scaffolds alone or loaded with hAECs were implanted into the alveolar defects of rats. Micro-computed tomography evaluation and histologic studies were conducted. Our results validated the in vitro osteogenic capacity of hAECs by upregulation of Runx2, osterix, alkaline phosphatase, collagen I, and osteopontin, with positive biochemical staining for osteoblasts. An epithelial-mesenchymal transformation process might be involved in the osteogenic differentiation of hAECs by increased expression of transforming growth factor-beta 1. Our data also demonstrated that in vivo implantation of hArCs loaded on beta-TCP scaffolds, not only improved bone regeneration by direct participation, but also reduced the early host immune response to the scaffolds. The presented data indicate that hAECs possess proper osteogenic differentiation potential and a modulatory influence on the early tissue remodeling process, making these cells a potential source of progenitor cells for clinical restoration of the alveolar defect.