THE DROSOPHILA SR PROTEIN RBP1 CONTRIBUTES TO THE REGULATION OF DOUBLESEX ALTERNATIVE SPLICING BY RECOGNIZING RBP1 RNA TARGET SEQUENCES
THE DROSOPHILA SR PROTEIN RBP1 CONTRIBUTES TO THE REGULATION OF DOUBLESEX ALTERNATIVE SPLICING BY RECOGNIZING RBP1 RNA TARGET SEQUENCES
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DOI：
10.1002/j.1460-2075.1995.tb00070.x
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发表时间：
1995-08-15
期刊：
影响因子：
11.4
通讯作者：
BAKER, BS
中科院分区：
文献类型：
--
作者：
HEINRICHS, V;BAKER, BS
SR蛋白代表了一个剪接因子家族，其中几个与果蝇中doublemex（dsx）前mRNA的性别特异性选择性剪接调控有关。dsx基因参与果蝇的性别决定。我们用体外筛选方法鉴定了果蝇SR蛋白RBP1识别的两个RNA靶序列基序。在dsx前体mRNA的两个对dsx选择性剪接调节重要的区域，即重复区和受调节的雌性特异性3'剪接位点的富含嘌呤的多聚嘧啶区，发现了这些RBP1靶序列的几个拷贝，我们表明，RBP1的dsx重复区域内的靶序列所需的dsx前mRNA的有效剪接。此外，我们的研究表明，RBP1有助于激活女性特异性dsx剪接在体内通过识别RBP1靶序列内的嘌呤丰富的多聚嘧啶道的女性特异性3'剪接位点。
The SR proteins represent a family of splicing factors several of which have been implicated in the regulation of sex-specific alternative splicing of doublesex (dsx) pre-mRNA in Drosophila. The dsx gene is involved in Drosophila sex determination. We have identified two RNA target sequence motifs recognized by the SR protein RBP1 from Drosophila using an in vitro selection approach, Several copies of these RBP1 target sequences were found within two regions of the dsx pre-mRNA which are important for the regulation of dsx alternative splicing, the repeat region and the purine-rich polypyrimidine tract of the regulated female-specific 3' splice site, We show that RBP1 target sequences within the dsx repeat region are required for the efficient splicing of dsx pre-mRNA. Moreover, our studies reveal that RBP1 contributes to the activation of female-specific dsx splicing in vivo by recognizing the RBP1 target sequences within the purine-rich polypyrimidine tract of the female-specific 3' splice site.