Genome-wide identification of replication origins in yeast by comparative genomics
Genome-wide identification of replication origins in yeast by comparative genomics
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DOI：
10.1101/gad.385306
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发表时间：
2006-07-15
影响因子：
10.5
通讯作者：
Donaldson, Anne D.
中科院分区：
文献类型：
--
作者：
Nieduszynski, Conrad A.;Knox, Yvonne;Donaldson, Anne D.
我们发现，在严格意义酵母菌中，复制起源功能所必需的序列往往是保守的。在这里，我们使用系统发育保守分析，以碱基对分辨率确定整个酿酒酵母基因组的复制起源序列。228个预测位点中的每一个都证实了起源活动，占表观起源区域的86%。这是第一个以足够的分辨率确定复制起始的全基因组位置的研究，以确定由复制蛋白结合的序列元件。我们的研究结果表明，系统发育保护可以用于鉴定负责复制真核生物基因组的起源序列。
We discovered that sequences essential for replication origin function are frequently conserved in sensu stricto Saccharomyces species. Here we use analysis of phylogenetic conservation to identify replication origin sequences throughout the Saccharomyces cerevisiae genome at base pair resolution. Origin activity was confirmed for each of 228 predicted sites - representing 86% of apparent origin regions. This is the first study to determine the genome-wide location of replication origins at a resolution sufficient to identify the sequence elements bound by replication proteins. Our results demonstrate that phylogenetic conservation can be used to identify the origin sequences responsible for replicating a eukaryotic genome.