QTL analysis for plant and grain characters of sake-brewing rice using a doubled haploid population
QTL analysis for plant and grain characters of sake-brewing rice using a doubled haploid population
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DOI：
10.1270/jsbbs.52.309
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发表时间：
2002-12-01
期刊：
影响因子：
2.4
通讯作者：
Kamijima, O
中科院分区：
文献类型：
--
作者：
Yoshida, S;Ikegami, M;Kamijima, O
用于酿造清酒的水稻（Oryza sativa L.）品种通常具有诸如大颗粒和白核（稻米内部不透明的结构）等特征。利用烹饪品种“Reiho”和清酒品种“Yamada-nishiki”杂交的双单倍体系构建了连锁图谱。采用随机扩增多态性DNA （RAPD）、扩增片段长度多态性（AFLP）和简单序列重复（SSR）标记系统进行QTL分析。在水稻染色体上共鉴定和定位了145个标记。采用区间作图和单点分析方法检测植株和籽粒性状的qtl。鉴定出了对清酒酿造重要性状有显著贡献的几个qtl，包括粒度、粒形、白核粒率和蛋白质含量。几个qtl同时影响粒重、粒宽和粒厚，而与粒长有关的qtl单独影响晶粒尺寸。白核粒率QTL对籽粒大小没有影响，而白腹粒率QTL同时影响粒重、粒宽和粒厚。在糙米和精米中均检测到多个蛋白质含量的qtl。4号染色体上一个降低精米蛋白质含量的QTL与籽粒长度呈正相关。11号染色体上对粒长影响最大的一个QTL没有导致精米蛋白质含量的降低。因此，第4染色体上的粒长QTL可能不仅控制了籽粒形状，还控制了与碾磨效率和/或储存蛋白位置相关的内部结构。
Rice (Oryza sativa L.) varieties used for brewing sake are commonly characterized by traits such as large grain size with white-core (an opaque structure inside the rice grain). A linkage map was constructed using doubled haploid lines derived from the cross of Reiho (a cooking variety) and Yamada-nishiki (a sake-brewing variety). Random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker systems were employed in QTL analysis. A total of 145 markers were identified and mapped on rice chromosomes. QTLs for plant and grain characters were detected by interval mapping and single point analysis. Several QTLs with a significant contribution were identified for important sake-brewing characters including grain size, grain shape, white-core grain rate and protein content. Several QTLs simultaneously affected the grain weight, width and thickness, while QTLs for the grain length independently affected the grain size. QTLs for the white-core grain rate did not affect the grain size, although one QTL for the white-belly grain rate simultaneously affected the grain weight, width and thickness. Several QTLs were detected for the protein content in both brown and polished rice. One QTL on chromosome 4 that was effective for the decrease of the protein content in polished rice showed a positive relation with the grain length. One QTL with the largest effect on the grain length on chromosome 11 did not contribute to the decrease of the protein content in polished rice. Therefore, it is suggested that the grain length QTL on chromosome 4 might control not only the grain shape but also the internal structure related to the milling efficiency and/or location of the storage protein.