The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells
The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells
复制标题
DOI：
10.1016/j.bbrc.2012.11.075
复制
发表时间：
2013-01-11
影响因子：
3.1
通讯作者：
Kuninaka, Shinji
中科院分区：
文献类型：
--
作者：
Naoe, Hideaki;Chiyoda, Tatsuyuki;Kuninaka, Shinji
胎盘滋养层干细胞（TS）向滋养层巨细胞（TG）的分化伴随着从有丝分裂细胞周期到内周期的转变。在这里，我们报告说，Cdh 1，调节后期促进复合物/细胞周期体（APC/C），负调控有丝分裂进入后，有丝分裂/内循环过渡。来自纯合Cdh 1基因捕获（Cdh 1（GT/GT））小鼠胚胎的TS细胞积累有丝分裂周期蛋白，并在诱导TS细胞分化后过早进入有丝分裂，表明Cdh 1是从有丝分裂到内循环的转换所必需的。此外，Cdh 1（GT/GT）TS细胞和胎盘显示异常表达胎盘分化标志物。这些数据突出了Cdh 1在胎盘分化过程中的G2/M转换中的重要作用。(C)2012 Elsevier Inc. All rights reserved.
Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1(GT/GT)) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1(GT/GT) TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation. (C) 2012 Elsevier Inc. All rights reserved.