Effects of Composition of Iron-Cross-Linked Alginate Hydrogels for Cultivation of Human Dermal Fibroblasts
Effects of Composition of Iron-Cross-Linked Alginate Hydrogels for Cultivation of Human Dermal Fibroblasts
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DOI：
10.1155/2012/820513
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Namiki, Hideo
中科院分区：
文献类型：
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作者：
Machida-Sano, Ikuko;Ogawa, Sakito;Namiki, Hideo
我们研究了铁离子交联海藻酸盐（Fe-alginate）与不同比例的l -古鲁醛酸(G)和d -甘露醛酸(M)残基作为人真皮成纤维细胞培养底物的适宜性。高g和高m海藻酸铁凝胶在促进细胞初始粘附和蛋白质吸附能力方面的效果相当，但随着培养时间的推移，高g海藻酸铁凝胶比高m海藻酸铁凝胶的细胞增殖能力更强。在培养液中浸泡期间，高浓度海藻酸铁的凝胶性能在溶胀和聚合物含量方面变化不大，但由于离子交联的损失，高浓度海藻酸铁凝胶的性能发生了变化。然而，高m海藻酸铁凝胶浸泡在培养基中稳定后，细胞增殖程度有所提高，直到不再发生变化。这些结果表明，铁离子与藻酸盐之间的交联模式因藻酸盐成分的不同而不同，导致两种类型的藻酸铁膜上细胞生长差异的主要因素是培养过程中的凝胶稳定性，而不是原始凝胶的膨胀、聚合物含量或蛋白质吸附能力。我们的研究结果可能有助于将海藻酸铁扩展到各种生物医学领域。
We investigated the suitability of ferric-ion-cross-linked alginates (Fe-alginate) with various proportions of L-guluronic acid (G) and D-mannuronic acid (M) residues as a culture substrate for human dermal fibroblasts. High-G and high-M Fe-alginate gels showed comparable efficacy in promoting initial cell adhesion and similar protein adsorption capacities, but superior cell proliferation was observed on high-G than on high-M Fe-alginate as culture time progressed. During immersion in culture medium, high-G Fe-alginate showed little change in gel properties in terms of swelling and polymer content, but the properties of high-M Fe-alginate gel were altered due to loss of ion cross-linking. However, the degree of cell proliferation on high-M Fe-alginate gel was improved after it had been stabilized by immersion in culture medium until no further changes occurred. These results suggest that the mode of cross-linkage between ferric ions and alginate differs depending on alginate composition and that the major factor giving rise to differences in cell growth on the two types of Fe-alginate films is gel stability during culture, rather than swelling of the original gel, polymer content, or protein adsorption ability. Our findings may be useful for extending the application of Fe-alginate to diverse biomedical fields.