A novel method for rapid detection of Streptococcus pneumoniae antigen in sputum and its application in adult respiratory tract infections
A novel method for rapid detection of Streptococcus pneumoniae antigen in sputum and its application in adult respiratory tract infections
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DOI：
10.1099/jmm.0.47793-0
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发表时间：
2008-07-01
影响因子：
3
通讯作者：
Kohn, Shigeru
中科院分区：
文献类型：
--
作者：
Ehara, Naomi;Fukushima, Kiyoyasu;Kohn, Shigeru
利用肺炎链球菌C-多糖部分的特异性多克隆抗体，研制了一种高灵敏度的免疫层析试剂盒ODKO 501，用于快速检测肺炎链球菌。痰液样本中的肺炎抗原。在52例成人呼吸道感染患者中前瞻性评价了ODKO 501检测的临床实用性，并与尿抗原检测试剂盒进行了比较。总体而言，21例患者（40.4%）ODKO 501显示阳性结果，相比之下，16例患者（30.8%）使用尿抗原检测试剂盒，和S。18例患者培养出肺炎克雷伯菌。ODKO 501和尿抗原检测试剂盒的敏感性分别为94.4%和55.6%（P
A highly sensitive immunochromatography test kit, ODKO501, was developed using specific polyclonal antibodies against the C-polysaccharide moiety of Streptococcus pneumoniae for the rapid detection of S. pneumoniae antigen in sputum samples. The clinical utility of ODKO501 for this detection was evaluated prospectively in 52 adult patients with respiratory infections and compared with that of a urinary antigen detection kit. Overall, 21 patients (40.4%) showed positive results with ODKO501, compared with 16 patients (30.8 %) using the urinary antigen detection kit, and S. pneumoniae was cultured from 18 patients. ODKO501 and the urinary antigen detection kit exhibited a sensitivity of 94.4 and 55.6% (P