Zebrafish as a model for infectious disease and immune function
Zebrafish as a model for infectious disease and immune function
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DOI：
10.1016/j.fsi.2008.05.005
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发表时间：
2008-10-01
影响因子：
4.7
通讯作者：
Kim, Carol H.
中科院分区：
文献类型：
--
作者：
Sullivan, Con;Kim, Carol H.
斑马鱼（Danio rerio）作为研究人类发育、神经生物学和遗传学的有力模型，已处于生物医学研究的前沿。近年来，斑马鱼系统的应用已扩展到行为、免疫学和毒理学研究领域，其作为人类疾病模型的理念得以保留。作为被研究得最为透彻的硬骨鱼之一，斑马鱼拥有丰富的遗传和基因组信息，目前正被视为研究有鳍鱼类病原体的模型。其基因组目前正在进行测序和注释，基因微阵列和插入突变体已有商业供应。通过吗啉代寡核苷酸进行基因特异性翻译敲低的应用十分广泛。因此，一些实验室已经利用斑马鱼建立了细菌和病毒疾病模型，以研究对感染的免疫反应。尽管许多斑马鱼病原体模型是为了解决人类传染病问题而建立的，但这些研究的结果应该为开发针对经济上重要的鱼类的细菌和病毒病原体的有效疫苗和预防措施提供重要线索。在这篇综述中，将讨论斑马鱼模型系统的能力和潜力，并对斑马鱼传染病模型的相关信息进行概述。（c）2008爱思唯尔有限公司。保留所有权利。
The zebrafish, Danio rerio, has come to the forefront of biomedical research as a powerful model for the study of development., neurobiology, and genetics of humans. In recent years, use of the zebrafish system has extended into studies in behaviour, immunology and toxicology, retaining the concept that it will serve as a model for human disease. As one of the most thoroughly studied teleosts, with a wealth of genetic and genomic information available, the zebrafish is now being considered as a model for pathogen studies in finfishes. Its genome is currently being sequenced and annotated, and gene microarrays and insertional mutants are commercially available. The use of gene-specific knockdown of translation through morpholino oligonucleotides is widespread. As a result, several laboratories have developed bacterial and viral disease models with the zebrafish to study immune responses to infection. Although many of the zebrafish pathogen models were developed to address human infectious disease, the results of these studies should provide important clues for the development of effective vaccines and prophylactic measures against bacterial. and viral pathogens in economically important fishes.In this review, the capabilities and potential of the zebrafish model system will be discussed and an overview of information on zebrafish infectious disease models will. be presented. (c) 2008 Elsevier Ltd. All rights reserved.