CLONAL CHARACTER OF F1 HYBRID LYMPHOCYTE SUBSET RECOGNITION OF PARENTAL CELLS IN ONE-WAY MIXED LYMPHOCYTE CULTURES
CLONAL CHARACTER OF F1 HYBRID LYMPHOCYTE SUBSET RECOGNITION OF PARENTAL CELLS IN ONE-WAY MIXED LYMPHOCYTE CULTURES
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单向混合淋巴细胞培养物中亲本细胞F1混合淋巴细胞亚群识别的克隆特征
DOI：
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发表时间：
1974
影响因子：
15.3
通讯作者：
Richard T. Smith
中科院分区：
文献类型：
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作者：
B. Gebhardt;Y. Nakao;Richard T. Smith
丝裂霉素阻断的亲代细胞刺激混合淋巴细胞培养物中 F1 杂交淋巴细胞的增殖。使用源自小鼠同源系的 F1 杂交体对这种现象的证明表明，刺激是由主要组织相容性基因座染色体区域中或与其密切相关的基因控制的。与荷瘤小鼠具有增强的主要同种异体抗原识别能力的发现相一致，观察到荷瘤小鼠对亲本的F1杂种反应也增强。对单向混合培养物中分裂细胞的染色体分析证实，F1 细胞（而不是受阻的亲代细胞）进入有丝分裂。没有观察到亲代细胞释放的可溶性介体对F1细胞的刺激，亲代细胞的丝裂霉素阻断似乎是完全有效的阻断剂，确保亲代细胞不能进入DNA合成。使用 5-溴脱氧尿苷自杀程序证明了 F1 识别亲本的特异性和克隆性质。在此类实验中，F1 脾细胞群中不同的淋巴细胞克隆似乎可以识别其中一个亲本，但不能同时识别两者。这些观察结果和肿瘤系统中的其他观察结果表明，大多数或所有杂合生物体可能拥有潜在的自我反应性淋巴细胞克隆。
Proliferation of F1 hybrid lymphocytes in mixed lymphocyte cultures is stimulated by mitomycin-blocked parental cells. The demonstration of this phenomenon using F1 hybrids derived from congenic lines of mice establishes that the stimulation is controlled by genes in or closely linked to the major histocompatibility locus chromosome region. In agreement with the finding that tumor-bearing mice have an increased capacity for primary alloantigen recognition, it was observed that the F1 hybrid response to parent was also augmented by tumor bearing. Chromosomal analysis of dividing cells in one-way mixed cultures confirms that F1 cells, and not the blocked parental cells, enter mitosis. Stimulation of F1 cells by a soluble mediator liberated by the parental cells was not observed and mitomycin blocking of parental cells seems to be a completely effective blocking agent ensuring that parental cells can not enter DNA synthesis. The specificity and clonal nature of F1 recognition of parent was demonstrated using a 5-bromodeoxyuridine-suicide procedure. Distinct clones of lymphocytes in F1 spleen cell populations seem to recognize one or the other parent, but not both, in such experiments. These observations and others in tumor systems suggest that most or all heterozygous organisms may possess potentially self-reactive clones of lymphocytes.
影响因子：
6.2
作者：
Hoffman,RA;Ascher,NL;Nelson,RD;Simmons,RL
通讯作者：
Simmons,RL