Identification of two phytotoxins, blumenol A and grasshopper ketone, in the allelopathic Japanese rice variety Awaakamai
Identification of two phytotoxins, blumenol A and grasshopper ketone, in the allelopathic Japanese rice variety Awaakamai
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DOI：
10.1016/j.jplph.2012.01.009
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发表时间：
2012-05-01
影响因子：
4.3
通讯作者：
Suenaga, Kiyotake
中科院分区：
文献类型：
--
作者：
Kato-Noguchi, Hisashi;Tamura, Kazuya;Suenaga, Kiyotake
日本传统水稻品种中具有最大化感作用的水稻品种Awaakami的甲醇水提取物对水芹（Lepidium sativum）、莴苣（Lactuca sativa）、梯牧草（Phosphorus pratense）、马唐（Digitaria sanguarum）、多花黑麦草（Lolium multiflorum）和稗草（Echinochloa crus-galli）的根和芽的生长具有抑制作用。随着提取物浓度的增加，抑制作用增强，表明淡泊茶提取物中含有生长抑制物质。对淡泊花提取物进行了分离纯化，分离出两种主要的生长抑制物质，并通过光谱数据确定为勃鲁米醇A和蚱蜢酮。当浓度大于10 μ mol/L和30 μ mol/L时，艾美醇A和蚱蜢酮分别抑制水芹芽和根的生长。对水芹根和芽的50%生长抑制所需的浓度分别为84和27 μ mol/L。蝗虫酮的浓度分别为185 μ mol/L和76 μ mol/L。这些结果表明，blumenol A和蚱蜢酮可能有助于Awaakami的生长抑制作用，并可能在Awaakami的化感作用中发挥重要作用。(C)2012 Elsevier GmbH. All rights reserved.
Aqueous methanol extracts of the traditional rice (Oryza sativa) variety Awaakamai, which is known to have the greatest allelopathic activity among Japanese traditional rice varieties, inhibited the growth of roots and shoots of cress (Lepidium sativum), lettuce (Lactuca sativa), timothy (Phleum pratense), Digitaria sanguinalis, Lolium multiflorum and Echinochloa crus-galli. Increasing the extract concentration increased the inhibition, suggesting that the extract of Awaakamai contains growth inhibitory substances. The extract of Awaakamai was purified and two main growth inhibitory substances were isolated and determined by spectral data as blumenol A and grasshopper ketone. Blumenol A and grasshopper ketone, respectively, inhibited the growth of cress shoots and roots at concentrations greater than 10 and 30 mu mol/L. The concentrations required for 50% growth inhibition on cress roots and shoots were 84 and 27 mu mol/L, respectively, for blumenol A. and 185 and 76 mu mol/L, respectively, for grasshopper ketone. These results suggest that blumenol A and grasshopper ketone may contribute to the growth inhibitory effect of Awaakamai and may play an important role in the allelopathy of Awaakamai. (C) 2012 Elsevier GmbH. All rights reserved.