Orexins increase cortical acetylcholine release and electroencephalographic activation through orexin-1 receptor in the rat basal forebrain during isoflurane anesthesia
Orexins increase cortical acetylcholine release and electroencephalographic activation through orexin-1 receptor in the rat basal forebrain during isoflurane anesthesia
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DOI：
10.1097/00000542-200605000-00019
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发表时间：
2006-05-01
期刊：
影响因子：
8.8
通讯作者：
Higuchi, T
中科院分区：
文献类型：
--
作者：
Dong, HI;Fukuda, S;Higuchi, T
背景：胆碱能唤醒系统在意识维持中起重要作用。作者研究了基底节内注射orexin-A或orexin-B和电刺激脚桥被盖核是否（PPTg：胆碱能上行通路的起源）可能改变异氟烷麻醉大鼠中与食欲素能系统相关的躯体感觉皮层中的乙酰胆碱流出和脑电图活动。将食欲素-A（10、30或100 pmol）或食欲素-B（10、30或100 pmol）（各n = 6）注射到基底前脑中，同时在1.0最小肺泡浓度（1.2%）异氟烷麻醉期间测量脑电图。注射林格氏液作为对照。在以下条件下对PPTg进行两次电刺激：（0.2 ms，100 Hz，400 μ A）/min持续20 min。在第二次PPTg刺激前20 min，将Ringer溶液或20 μ g SR 334867（一种食欲素-1受体拮抗剂结果：注射orexin-A（30和100 pmol）和orexin-B（100 pmol）可显著增加躯体感觉皮层的乙酰胆碱流出（P <0.05）。注射orexin-A（10、30、100 pmol）和orexin-B（30、100 pmol）改变了觉醒脑电图的爆发和抑制模式。与orexin-B相比，注射低剂量orexin-A可引起乙酰胆碱流出和唤醒脑电图的增加。结论：orexin-A比orexin-B能更有效地引起基底前脑胆碱能神经元活动的改变，orexin-I受体可能介导了PPTg刺激引起的皮层激活。
Background: Cholinergic arousal system plays an important role in the maintenance of consciousness. The authors investigated whether the intrabasalis injection of orexin-A or orexin-B and the electrically stimulated pedunculopontine tegmentum nuclei (PPTg: the origin of cholinergic ascending pathways) may alter acetylcholine efflux and electroencephalographic activity in the somatosensory cortex in relation to the orexinergic system in isoflurane-anesthetized rats.Methods: Either orexin-A (10, 30, or 100 pmol) or orexin-B (10, 30, or 100 pmol) (n = 6 each) was injected into the basal forebrain while the electroencephalogram was measured during 1.0 minimum alveolar concentration (1.2%) isoflurane anesthesia. Injection of Ringer's solution was used as a control. The PPTg was electrically stimulated twice with the following conditions: 1-s stimulus train (0.2 ms, 100 Hz, 400 mu A) per min for 20 min. Twenty minutes before the second PPTg stimulation, Ringer's solution or 20 mu g SR334867, an orexin-1 receptor antagonist (n = 5 each) was injected into the basal forebrain.Results: Injection of orexin-A (30 and 100 pmol) and orexin-B (100 pmol) significantly increased the acetylcholine efflux in the somatosensory cortex (P < 0.05). Injection of orexin-A (10, 30, 100 pmol) and orexin-B (30, 100 pmol) changed the burst and suppression patterns to arousal electroencephalogram. Compared with orexin-B, injection of a lower dose of orexin-A induced increase in the acetylcholine efflux and arousal electroencephalogram. SB334867 significantly attenuated the increases in the acetylcholine efflux and electroencephalographic activation evoked by PPTg stimulation.Conclusion: The authors demonstrated that orexin-A was more potent than orexin-B in producing alteration of cholinergic basal forebrain neuronal activity and that the cortical activation induced by the PPTg stimulation against isoflurane anesthesia may he mediated through the orexin-l receptors in the basal forebrain.