Gamma interferon (IFN gamma) and IFN alpha/beta suppress murine myeloid colony formation (CFU-C)N: magnitude of suppression is dependent upon level of colony-stimulating factor (CSF).
Gamma interferon (IFN gamma) and IFN alpha/beta suppress murine myeloid colony formation (CFU-C)N: magnitude of suppression is dependent upon level of colony-stimulating factor (CSF).
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DOI：
10.4049/jimmunol.129.1.76
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发表时间：
1982-07
影响因子：
4.4
通讯作者：
G. Klimpel;W. R. Fleischmann;K. Klimpel
中科院分区：
文献类型：
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作者：
G. Klimpel;W. R. Fleischmann;K. Klimpel
使用 C57BL/6 小鼠的骨髓细胞研究了不同类型的干扰素 (IFN) 对体外巨噬细胞和粒细胞集落形成 (CFU-C) 的影响。使用标准甲基纤维素培养系统，以 L 细胞条件培养基 (LCCM) 作为集落刺激因子活性 (CSF) 来源，在第 7 天测量 CFU-C 形成，IFN γ 从葡萄球菌肠毒素 A (SEA) 刺激的小鼠脾细胞中获得，并使用亲和层析进行部分纯化（50 倍）。 IFN α/β 获自新城疫病毒感染的小鼠 L 细胞，并使用 AcA 202 柱进行部分纯化（286 倍）。 IFN γ 以剂量依赖性方式抑制 CFU-C 形成，其抑制作用比 IFN α/β 强 100 倍。 IFN γ 完全抑制了 CFU-C 的形成，抗病毒活性低至 15 个单位。相反，需要1000至2000单位的IFNα/β抗病毒活性才能完全抑制CFU-C形成。经过pH 2处理（24小时）、热处理（60℃，2小时）或用针对IFNγ的特异性抗血清处理后，IFNγ抑制CFU-C形成的能力完全消失，这表明IFNγ是CFU-C抑制的介质。当IFNγ和IFNα/β混合在一起并添加到骨髓细胞中时，它们对抑制CFU-C形成具有协同作用。 IFN γ 和 IFN α/β 介导的抑制水平完全取决于培养系统中存在的 CSF 量；增加CSF水平可以完全克服IFN抑制作用。这些结果表明 IFN γ 和 IFN α/β 可能在造血过程中具有调节作用。
The effect of different types of interferon (IFN) on macrophage and granulocyte colony formation (CFU-C) in vitro was studied using bone marrow cells from C57BL/6 mice. CFU-C formation was measured at 7 days using a standard methylcellulose culture system with L-cell conditioned medium (LCCM) as a source of colony-stimulating factor activity (CSF), IFN gamma was obtained from staphylococcal enterotoxin A- (SEA) stimulated mouse spleen cells and was partially purified (50-fold) using affinity chromatography. IFN alpha/beta was obtained from Newcastle disease virus-infected mouse L-cells and was partially purified (286-fold) using an AcA 202 column. IFN gamma inhibited CFU-C formation in a dose-dependent manner and was 100 times more inhibitory than IFN alpha/beta. IFN gamma completely suppressed CFU-C formation with as little as 15 units of antiviral activity. In contrast, 1000 to 2000 units of antiviral activity of IFN alpha/beta was required for complete suppression of CFU-C formation. The ability of IFN gamma to inhibit CFU-C formation was completely abolished after pH 2 treatment (24 hr), heat treatment (60 degrees C, 2 hr), or treatment with a specific antiserum against IFN gamma, which indicated that IFN gamma was the mediator of CFU-C inhibition. When IFN gamma and IFN alpha/beta were mixed together and added to bone marrow cells, they had a synergistic effect on the suppression of CFU-C formation. The level of inhibition mediated by both IFN gamma and IFN alpha/beta was totally dependent upon the amount of CSF present in the culture system; increasing the level of CSF could completely overcome the IFN suppressive effect. These results suggest that IFN gamma and IFN alpha/beta may have regulatory roles in hematopoiesis.