Transgenic expression of Pinellia ternata agglutinin (PTA) and Arisaema heterophyllum agglutinin (AHA) in wheat confers resistance against the grain aphid, Sitobion miscanthi
Transgenic expression of Pinellia ternata agglutinin (PTA) and Arisaema heterophyllum agglutinin (AHA) in wheat confers resistance against the grain aphid, Sitobion miscanthi
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小麦中半夏凝集素 (PTA) 和天南星凝集素 (AHA) 的转基因表达赋予对谷蚜 Sitobion miscanthi 的抗性
DOI：
10.1007/s10340-021-01346-7
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发表时间：
2021-02-16
影响因子：
4.8
通讯作者：
Chen，Julian
中科院分区：
文献类型：
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作者：
Zhang，Yong;Deng，Qing;Chen，Julian
本研究以转半夏凝集素（PTA）和异叶天南星凝集素（AHA）基因的小麦品系为材料，研究了麦长管蚜（Sitobionmiscanthin）的取食行为和生命表参数，结果表明，转半夏凝集素和异叶天南星凝集素基因的小麦品系对麦长管蚜的取食行为有负面影响。取食PTA和AHA转基因小麦品系的蚜虫比取食非转基因品系的蚜虫具有更长的总非探测（np）期，但表现出更短的流涎期（E1）、韧皮部汁液摄取期（E2）和持续摄取期（E2 > 10 min）。此外，蚜虫喂养的171行，其中表示PTA，显示了显着增加的NP模式的数量和所需的时间，以启动第一个探针。此外，S.发现在任何表达PTA和AHA的小麦品系上的芒刺取食显著减少。蚜虫在AHA基因表达小麦上的最大繁殖力显著低于对照。取食PTA和AHA转基因小麦的蚜虫的净繁殖率（R0）和内禀增长率（rm）也显著低于取食非转化对照植株的蚜虫。这些发现表明成功检测到了S的耐药性。本发明的目的是通过在表达PTA和AHA的小麦品系中引入芒硫素，通过表达药用植物中天然存在的独特蛋白质，为针对作物害虫的工程保护提供新的选择。
The present study investigated the feeding behaviour and life-table parameters of the grain aphidSitobion miscanthiin response to being fed on transgenic wheat lines expressingPinellia ternataagglutinin (PTA) andArisaema heterophyllumagglutinin (AHA), both of which originate from Chinese medicinal plants.The findings revealed that the feeding behaviour ofS.miscanthion transgenic wheat lines was negatively affected. The aphids that feed on the PTA and AHA transgenic wheat lines had longer total non-probing (np) periods than the aphids that feed on non-transformed lines but exhibited shorter durations of salivation (E1), phloem sap ingestion phase (E2) and sustained ingestion (E2 > 10 min). Moreover, aphids feeding on the 171 line, which expresses PTA, displayed a significant increase in the number of np patterns and time taken to initiate the first probe. Furthermore, the lifespan ofS. miscanthifeeding on any of the wheat lines expressing PTA and AHA was found to reduce significantly. The maximum fecundity of the aphids on the AHA-expressing wheat lines was significantly lower than that of the control group. The net reproductive rate (R0) and intrinsic rate of increase (rm) were also significantly lower for the aphids feeding on the PTA and AHA transgenic wheat than for the aphids feeding on the non-transformed control plants. These findings indicate successful detection of resistance toS. miscanthiin the PTA- and AHA-expressing wheat lines, providing a new option for engineering protection against crop pests by expressing unique proteins that occur naturally in medicinal plants.