Transplacental effects of gentamicin on endocytosis in rat renal proximal tubule cells
Transplacental effects of gentamicin on endocytosis in rat renal proximal tubule cells
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庆大霉素经胎盘对大鼠肾近曲小管细胞内吞作用的影响
DOI：
10.1007/bf00856528
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发表时间：
1994
影响因子：
3
通讯作者：
J. Schaeverbeke
中科院分区：
文献类型：
--
作者：
H. Smaoui;M. Schaeverbeke;J. Mallié;J. Schaeverbeke
妊娠大鼠接受庆大霉素给药后，已报告子宫内肾脏成熟的变化。虽然通常观察到的蛋白尿可能与肾小球基底膜的改变有关，但近端肾小管细胞的变化可解释肾蛋白处理紊乱。因此，我们研究了庆大霉素对肾脏的处理和近端肾小管细胞中的蛋白质运输的影响，使用辣根过氧化物酶，液相标记物，作为探针。对妊娠Wistar大鼠腹腔注射庆大霉素（75 mg/kg体重/天），并在出生后1天研究新生大鼠肾脏。在近端肾小管细胞的深层皮质区，包含完全成熟的新生儿肾单位，运输和消化的重吸收过氧化物酶显着减少与对照相比，过氧化物酶到达溶酶体后的内吞作用。给药动物的尿蛋白排泄增加。我们的结论是，庆大霉素，进入近端肾小管细胞通过内吞途径，减少蛋白质的肾小管重吸收，从而增加尿蛋白排泄。
Changes in kidney maturation in utero have been reported after gentamicin administration to pregnant rats. While the proteinuria commonly observed, could be related to modifications of the glomerular basement membrane, perturbed renal protein handling could be accounted for by changes in the proximal tubular cells. Therefore, we studied the effect of gentamicin on the renal handling and transport of proteins in proximal tubular cells using the horseradish peroxidase, a fluid-phase marker, as a probe. Gentamicin was administered intraperitoneally to pregnant Wistar rats (75 mg/kg body weight per day) and neonatal kidneys were studied 1 day after birth. In proximal tubular cells of the deep cortical area, containing the fully matured nephrons of neonates, the transport and digestion of reabsorbed peroxidase was considerably reduced compared with controls where peroxidase reached lysosomes after endocytosis. Urinary protein excretion increased in treated animals. We conclude that gentamicin, entering the proximal tubular cells via the endocytic pathway, decreases the tubular reabsorption of proteins, thus increasing urinary protein excretion.
氨基糖苷类药物可诱导大鼠肾皮质中的磷脂沉积：肾毒性的早期表现。
DOI：
--
发表时间：
1982
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Feldman,S;Wang,MY;Kaloyanides,GJ
通讯作者：
Kaloyanides,GJ