Rap2B promotes migration and invasion of human suprarenal epithelioma.
Rap2B promotes migration and invasion of human suprarenal epithelioma.
复制标题
Rap2B促进人肾上上皮瘤的迁移和侵袭
DOI：
10.1007/s13277-014-2174-8
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Zheng JN
中科院分区：
文献类型：
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作者：
Di JH;Qu DB;Lu Z;Li LT;Cheng Q;Xin Y;Zhang LZ;Zhang Y;Zheng JN
本研究的目的是阐明Rap2B在人肾上皮瘤发生发展中的作用，并研究Rap2B对肾上皮瘤细胞迁移和侵袭的影响。我们使用组织微阵列和免疫组织化学来评估 75 例肾上皮瘤组织和 75 例肿瘤邻近正常肾组织中的 Rap2B 染色。同时Western blot检测人肾上皮瘤细胞和组织中Rap2B蛋白的表达。采用伤口愈合实验、细胞迁移实验、基质胶侵袭实验检测Rap2B在肾上皮瘤细胞迁移和侵袭中的作用。之后，我们进行了蛋白质印迹分析和明胶酶谱分析来检测MMP-2蛋白表达和酶活性。我们的研究表明，与肿瘤邻近的正常肾组织相比，肿瘤组织中 Rap2B 的表达增加。但未发现Rap2B表达与临床病理参数之间存在相关性。此外，我们发现Rap2B促进细胞迁移和侵袭能力，并且Rap2B增加肾上皮瘤细胞中MMP-2的表达和酶活性。我们的数据表明，Rap2B在人肾上皮瘤中表达显着增加，并且Rap2B可以促进细胞迁移和侵袭能力，这可能为肾上上皮瘤的治疗提供新的靶点。
The aim of our study was to elucidate the role of Rap2B in the development of human suprarenal epithelioma and to investigate the effect of Rap2B on suprarenal epithelioma cells migration and invasion. We use tissue microarray and immunohistochemistry to evaluate Rap2B staining in 75 suprarenal epithelioma tissues and 75 tumor-adjacent normal renal tissues. And the expression of Rap2B protein in human suprarenal epithelioma cells and tissues was detected by western blot simultaneously. The role of Rap2B in suprarenal epithelioma cells migration and invasion was detected by using wound healing assay, cell migration assay, and matrigel invasion assay. After that, we performed western blot analysis and gelatin zymography to detect MMP-2 protein expression and enzyme activity. Our research showed that Rap2B expression was increased in tumor tissues compared with tumor-adjacent normal renal tissues. But no correlation was found between Rap2B expression and clinicopathological parameters. In addition, we found that Rap2B promoted the cell migration and invasion abilities, and Rap2B increased MMP-2 expression and enzyme activity in suprarenal epithelioma cells. Our data indicated that Rap2B expression is significantly increased in human suprarenal epithelioma and Rap2B can promote the cell migration and invasion abilities, which may provide a new target for the treatment of suprarenal epithelioma.