Influence of extracts of Stryphnodendron polyphyllum Mart. and Stryphnodendron obovatum Benth. on the cicatrisation of cutaneous wounds in rats
Influence of extracts of Stryphnodendron polyphyllum Mart. and Stryphnodendron obovatum Benth. on the cicatrisation of cutaneous wounds in rats
复制标题
DOI：
10.1016/j.jep.2005.02.019
复制
发表时间：
2005-06-03
影响因子：
5.4
通讯作者：
de Mello, JCP
中科院分区：
文献类型：
--
作者：
Lopes, GC;Sanches, ACC;de Mello, JCP
本文报道了两种番木鳖的茎皮。和倒卵叶马钱子（Stryphnodendron obovatum Benth.），研究了豆科植物的伤口愈合、抗菌和抗氧化活性。这些植物的茎皮中分别含有12%和19%的单宁，在巴西被广泛用于传统医学。倒卵叶马钱子粗提物总酚含量为76.95 ± 2.98%（CV = 3.87%），乙酸乙酯级分（EAF）的CV为89.13 +/- 0.34%。（CV = 0.38%）;而在多叶马钱子中CE酚类化合物含量为51.62 +/- 1.53%（CV = 2.96%），EAF酚类化合物含量为59.00 +/- 1.91%（CV = 3.24%）。倒卵叶马钱子CE的单宁含量[36.58 ± 0.35%（CV = 0.98%）]比多叶马钱子CE的单宁含量[25.43 ± 0.96%（CV = 3.77%）]高11%左右。在EAF中，物种之间的差异甚至更大：在倒卵叶马钱子中，EAF酚含量为55.01 +/- 0.36%（CV = 0.65%），而在多叶马钱子中，含量为36.16 +/- 0.42%（CV = 1.16%）。在治疗4、7和10天后，研究了含有2.5%多叶马钱子和倒卵马钱子茎皮粗冻干提取物（PCE）和2.5%乙酸乙酯冻干部分（PEA）的软膏对Wistar（（R））大鼠皮肤伤口的治疗效果。通过计数硫酸长春新碱阻断的中期分裂相，评价伤口再上皮化区域的上皮细胞增殖。用PCE，在用多叶马钱子处理4天和7天后，以及用倒卵状马钱子处理7天和10天后，观察到表皮生长的增加。用倒卵叶马钱子PEA处理的伤口仅在处理后4天显示出表皮生长增加，对于多叶马钱子，在处理后4天和7天观察到表皮生长。多叶马钱子和倒卵马钱子的CE和EAF级分均显示出对金黄色葡萄球菌的抗菌活性，MIC值分别为125和250 μ g/ml。浓度> 1000 μ g/ml的提取物和级分不抑制测试的革兰氏阴性菌。通过TLC中DPPH自由基的还原的抗氧化活性，证实了这些提取物在两个物种中的抗自由基特性。这两个物种的CE和EAF表现出自由基清除活性（RSA）和保护DPPH变色，已经在0.032 μ g/ml。多叶马钱子的提取物比倒卵叶马钱子的提取物更有效，尽管前者的单宁含量较低。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Stem bark of the two species Stryphnodendron polyphyllum Mart. and Stryphnodendron obovatum Benth., Leguminosae, was investigated for wound healing, antibacterial and antioxidant activity. These plants contain 12 and 19% tannins in their stem bark, respectively, and are widely used in traditional medicine in Brazil. The total content of phenolics of the crude extract (CE) of Stryphnodendron obovatum was 76.95 +/- 2.98% (CV = 3.87%) and of the ethyl-acetate fraction (EAF) was 89.13 +/- 0.34% (CV = 0.38%); whereas in Stryphnodendron polyphyllum the CE phenolics content was 51.62 +/- 1.53% (CV = 2.96%) and the EAF phenolics content was 59.00 +/- 1.91% (CV = 3.24%). The tannin content of CE from Stryphnodendron obovatum [36.58 +/- 0.35% (CV = 0.98%)] was about 11% higher than in CE from Stryphnodendron polyphyllum [25.43 +/- 0.96% (CV = 3.77%)]. The difference between the species was even greater in the EAF: in Stryphnodendron obovatum the EAF phenolics content was 55.01 +/- 0.36% (CV = 0.65%), whereas in Stryphnodendron polyphyllum the content was 36.16 +/- 0.42% (CV = 1.16%). The healing effect of ointments containing 2.5% crude lyophilised extract (PCE) and 2.5% ethyl-acetate lyophilised fraction (PEA) of the stem bark of Stryphnodendron polyphyllum and Stryphnodendron obovatum was studied in cutaneous wounds of Wistar((R)) rats after 4, 7 and 10 days of treatment. Epithelial cell proliferation in the area of re-epithelialisation of the wounds was evaluated by counting the metaphases blocked by vincristine sulfate. With PCE an increase in epidermal growth was observed after 4 and 7 days of treatment with Stryphnodendron polyphyllum, and after 7 and 10 days of treatment with Stryphnodendron obovatum. Wounds treated with PEA of Stryphnodendron obovatum showed increased epidermal growth only 4 days after the treatment, for Stryphnodendron polyphyllum, epidermal growth was observed after 4 and 7 days of treatment. Both the CE and the EAF fractions of Stryphnodendron polyphyllum and Stryphnodendron obovatum showed antibacterial activity against Staphylococcus aureus with MIC values of 125 and 250 mu g/ml, respectively. Gram-negative bacteria tested were not inhibited by extracts and fractions at concentrations > 1000 mu g/ml. The antioxidant activity through reduction of the DPPH radical in TLC, confirmed the anti-radical properties of these extracts in both species. CE and EAF of both species showed a radical scavenging activity (RSA) and protected DPPH from discolouration, already at 0.032 mu g/ml. The extract from Stryphnodendron polyphyllum were more effective than those Stryphnodendron obovatum, although the former had a lower tannin content. (c) 2005 Elsevier Ireland Ltd. All rights reserved.