Aberration-corrected cryogenic objective mirror with a 0.93 numerical aperture
Aberration-corrected cryogenic objective mirror with a 0.93 numerical aperture
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DOI：
10.1063/1.5110546
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发表时间：
2019-07
影响因子：
4
通讯作者：
M. Fujiwara;T. Ishii;Keita Ishida;Y. Toratani;Taku Furubayashi;M. Matsushita;S. Fujiyoshi
中科院分区：
文献类型：
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作者：
M. Fujiwara;T. Ishii;Keita Ishida;Y. Toratani;Taku Furubayashi;M. Matsushita;S. Fujiyoshi
我们研制了一种低温物镜[Toratani-Fujiwara （TORA-FUJI）镜]，视场半径为36 μm，数值孔径为0.93。最新报道的超氦浸泡条件下的低温物镜（INAGAWA镜）具有接近最大数值孔径（0.99）和完全消色差。但视场半径限制在1.5 μm以内，这主要是由于彗差造成的。在TORA-FUJI反射镜中，校正彗差实现了36 μm视场半径。此外，对剩余的四种赛德尔像差和色差进行了充分的校正。为了评估光学性能，使用TORA-FUJI反射镜在685 nm激发波长下对单个染料进行低温荧光成像。结果表明，在1.8 K的超流氦条件下，TORA-FUJI反射镜在视场区域表现出接近衍射极限的性能。我们研制了一种低温物镜[Toratani-Fujiwara （TORA-FUJI）镜]，视场半径为36 μm，数值孔径为0.93。最新报道的超氦浸泡条件下的低温物镜（INAGAWA镜）具有接近最大数值孔径（0.99）和完全消色差。但视场半径限制在1.5 μm以内，这主要是由于彗差造成的。在TORA-FUJI反射镜中，校正彗差实现了36 μm视场半径。此外，对剩余的四种赛德尔像差和色差进行了充分的校正。为了评估光学性能，使用TORA-FUJI反射镜在685 nm激发波长下对单个染料进行低温荧光成像。结果表明，在1.8 K的超流氦条件下，TORA-FUJI反射镜在视场区域表现出接近衍射极限的性能。
We developed a cryogenic objective mirror [Toratani-Fujiwara (TORA-FUJI) mirror] with a 36-μm field of view (FOV) radius and a 0.93 numerical aperture. The latest reported cryogenic objective mirror (INAGAWA mirror) under a superfluid-helium immersion condition had a nearly maximum numerical aperture (0.99) and was perfectly achromatic. However, its FOV radius was restricted to 1.5 μm, mainly due to coma aberration. In the TORA-FUJI mirror, correcting coma aberration realized the 36-μm FOV radius. In addition, the remaining four Seidel aberrations and the chromatic aberrations were sufficiently corrected. To evaluate the optical performance, the cryogenic fluorescence imaging of individual dyes was performed with the TORA-FUJI mirror at a 685-nm excitation wavelength. This result shows that the TORA-FUJI mirror in superfluid helium at 1.8 K exhibits nearly diffraction-limited performance in the FOV region.We developed a cryogenic objective mirror [Toratani-Fujiwara (TORA-FUJI) mirror] with a 36-μm field of view (FOV) radius and a 0.93 numerical aperture. The latest reported cryogenic objective mirror (INAGAWA mirror) under a superfluid-helium immersion condition had a nearly maximum numerical aperture (0.99) and was perfectly achromatic. However, its FOV radius was restricted to 1.5 μm, mainly due to coma aberration. In the TORA-FUJI mirror, correcting coma aberration realized the 36-μm FOV radius. In addition, the remaining four Seidel aberrations and the chromatic aberrations were sufficiently corrected. To evaluate the optical performance, the cryogenic fluorescence imaging of individual dyes was performed with the TORA-FUJI mirror at a 685-nm excitation wavelength. This result shows that the TORA-FUJI mirror in superfluid helium at 1.8 K exhibits nearly diffraction-limited performance in the FOV region.