HPV genotype prevalence in Australian women undergoing routine cervical screening by cytology status prior to implementation of an HPV vaccination program
HPV genotype prevalence in Australian women undergoing routine cervical screening by cytology status prior to implementation of an HPV vaccination program
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DOI：
10.1016/j.jcv.2014.04.013
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发表时间：
2014-07-01
影响因子：
8.8
通讯作者：
Garland, Suzanne M.
中科院分区：
文献类型：
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作者：
Tabrizi, Sepehr N.;Brotherton, Julia M. L.;Garland, Suzanne M.
背景：在澳大利亚，按年龄和细胞学异常级别划分的宫颈HPV基因分型的患病率的数据很少。目的：根据年龄和细胞学状况测量2620名澳大利亚妇女中HPV基因分型的患病率。研究设计：参加常规巴氏涂片筛查的妇女来自不同地区，包括大量土著妇女样本。用罗氏线阵HPV对ThinPrep标本的DNA提取液进行HPV基因分型。结果：HPV的感染率和基因分布按年龄(平均32.6岁)和巴氏涂片结果(细胞学正常率86.7%)进行分层。HPV总患病率为38.7%，其中高危型HPV患病率为26.5%。随着年龄的增长，HPV(40岁女性66.3%)、多重HPV感染(40岁45.5%)和疫苗靶基因(HPV6/11/16/18)(34.1%)的患病率显著下降。HPV16(8.3%)、51(5.1%)、53(4.7%)、62(4.3%)、89(3.9%)和52(3.8%)。高危人乳头瘤病毒感染率从细胞学正常的妇女的21.1%增加到细胞学预测高度异常(HGA)妇女的80.9%(p<；0.001)。HGAs妇女中最常见的HPV16(51.1%)、18(14.9%)、52(12.8%)、31(10.6%)和33(10.6%)：全部为HR型。HPV16是所有年龄段和细胞学分级中最常见的高危类型。(C)2014年爱思唯尔BAT。版权所有。
Background: Data on the prevalence of cervical HPV genotypes in Australia by age and by grade of cytological abnormality are sparse.Objective: Measure prevalence of HPV genotypes among 2620 Australian women by age and cytology status.Study design: Women presenting for routine Pap smear screening were recruited from diverse regions, including a significant sample of Indigenous women. DNA extracts prepared from Thinprep specimens were HPV genotyped by Roche LINEAR ARRAY HPV.Results: HPV prevalence and genotype distribution were stratified by age (mean 32.6y) and Pap smear result (cytology normal in 86.7%). Overall HPV prevalence was 38.7% with high-risk HPV prevalence of 26.5%. Prevalence of HPV (66.3% in women 40 y), multiple HPV infection (45.5% in 40y) and vaccine-targeted genotypes (HPV 6/11/16/18) (34.1% in 40y) declined significantly with age. The six most common genotypes were: HPV 16(8.3%), 51 (5.1%), 53(4.7%), 62(4.3%), 89(3.9%) and 52(3.8%). HR-HPV prevalence increased from 21.1% in women with normal cytology to 80.9% in those with cytologically predicted high-grade abnormalities (HGAs) (p < 0.001). The most common genotypes in women with HGAs were HPV 16 (51.1%), 18 (14.9%), 52 (12.8%), 31(10.6%), and 33(10.6%): all HR-types.Conclusion: Pre-vaccination cross-sectional prevalence of HR-HPV infection was high in this sample of Australian women attending for screening. HPV 16 was the commonest high-risk type detected at all ages and cytological grades. (C) 2014 Elsevier BAT. All rights reserved.