Organ-specific regulation of the CD8 T cell response to Listeria monocytogenes infection
Organ-specific regulation of the CD8 T cell response to Listeria monocytogenes infection
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DOI：
10.4049/jimmunol.166.5.3402
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发表时间：
2001-03-01
影响因子：
4.4
通讯作者：
Lefrançois, L
中科院分区：
文献类型：
--
作者：
Pope, C;Kim, SK;Lefrançois, L
使用 MHC I 类四聚体测量肠粘膜 CD8 T 细胞对单核细胞增生李斯特菌感染的反应，并与外周血、次级淋巴组织和肝脏中的反应进行比较。为了评估李斯特菌的疫苗接种潜力并分析 C57BL/6 小鼠品系的反应，产生了表达 OVA (rLM-ova) 的重组李斯特菌。与脾脏相比，反应在感染后 9 天达到峰值，肠粘膜和肝脏 CD8 T 具有 OVA 特异性，比例要大得多。然而，这些差异与每个位点的细菌滴度无关。固有层和肝脏的较高反应导致这些组织中存在较大的 CD8 记忆群体。此外，记忆诱发的mas水平取决于感染剂量，并与口服攻击后回忆反应的强度呈负相关，组织中的回忆反应在固有层和肝脏中最为强烈，并且重新激活的Ag特异性T细胞产生IFN-γ。CD40或MHC II类缺陷小鼠的感染诱导了肠粘膜中较差的CD8 T细胞反应， 但仅部分降低了脾脏和肝脏的反应。总体而言，结果指出了组织特异性调节初级和记忆抗菌 CD8 T 细胞反应的新途径。
The intestinal mucosal CD8 T cell response to infection with Listeria monocytogenes tvas measured using MHC class I tetramers and was compared with the response in peripheral blood, secondary lymphoid tissue, and liver. To assess the vaccination potential of Listeria and to analyze responses in C57BL/6 mouse strains, a recombinant Listeria expressing OVA (rLM-ova) was generated. The response peaked at 9 days postinfection with a much larger fraction of the intestinal mucosa and liver CD8 T fell pool OVA specific, as compared with the spleen. However, these differences were not linked to bacterial titers in each site. The higher responses in lamina propria and liver resulted in a larger CD8 memory population in these tissues. Furthermore, the level of memory induced mas dependent on infectious dose and inversely correlated with the magnitude of the recall response after oral challenge, Recall responses in the tissues were most robust in the lamina propria and liver, and reactivated Ag-specific T cells produced IFN-gamma, Infection of CD40- or MHC class II-deficient mice induced poor CD8 T cell responses in the intestinal mucosa, but only partially reduced responses in the spleen and liver. Overall, the results point to novel pathways of tissue specific regulation of primary and memory antimicrobial CD8 T cell responses.