In vitro adhesion assays for probiotics and their in vivo relevance: A review.
In vitro adhesion assays for probiotics and their in vivo relevance: A review.
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DOI：
10.1080/08910600310019886
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发表时间：
2003-12-01
影响因子：
--
通讯作者：
Salminen, Seppo
中科院分区：
文献类型：
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作者：
Ouwehand, Arthur C.;Salminen, Seppo
对肠粘膜的粘附性是益生菌的主要选择标准之一。在体外进行粘附菌株的选择。然而，关于体外粘附与体内粘附的相关性知之甚少;迄今为止，尚未对这一点进行系统综述。由于各种粘连模型通常仅描述肠粘膜的一部分，因此模型的组合可能提供最佳信息。孵育条件影响粘附试验的结果，应根据生理相关性进行优化。消化、食物基质和正常微生物群的影响似乎有显着的影响，但尚未得到广泛的研究。许多因素会干扰益生菌的粘膜粘附，因此很难将体外粘附结果可靠地外推到人体体内情况。因此，体外和体内粘附之间的关系以及粘附对益生菌功效的意义值得进一步研究。在确定体内粘附的重要性以及体外和体内粘附之间的假定关系时，粘附和低粘附等基因菌株似乎是最好的工具。
Adhesion to the intestinal mucosa is one of the main selection criteria for probiotics. Selection of adhesive strains is performed in vitro. Little is known, however, as to how well in vitro adhesion correlates with in vivo adhesion; to date, this has not been systematically reviewed. Because the various adhesion models usually describe only one part of the intestinal mucosa, a combination of models may provide the best information. Incubation conditions affect the outcome of an adhesion assay and should be optimized for physiological relevance. The effects of digestion, the food matrix and the normal microbiota appear to have a significant influence, but have not been extensively investigated. Many factors interfere with the mucosal adhesion of probiotics, it is therefore difficult to extrapolate in vitro adhesion results reliably to the in vivo situation in humans. It is suggested that the relationship between in vitro and in vivo adhesion and the significance of adhesion for probiotic efficacy should be further investigated. In determining the in vivo importance of adhesion and the assumed relationship between in vitro and in vivo adhesion, adhesive and low-adhesive isogenic strains would appear to be the best tools.