Decidual and peripheral blood CD4+CD25+ regulatory T cells in early pregnancy subjects and spontaneous abortion cases
Decidual and peripheral blood CD4+CD25+ regulatory T cells in early pregnancy subjects and spontaneous abortion cases
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DOI：
10.1093/molehr/gah044
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发表时间：
2004-05-01
影响因子：
4
通讯作者：
Saito, S
中科院分区：
文献类型：
--
作者：
Sasaki, Y;Sakai, M;Saito, S
人类妊娠是一种半异体移植到母体宿主的情况。因此，有报道称，对同种异体胎儿移植的耐受是维持妊娠的一种机制。已知的是，CD4(+)CD25(Bright)调节性T细胞在外周组织耐受的形成和维持中发挥重要作用。然而，CD4(+)CD25(Bright)T细胞在维持人类妊娠中的潜在作用尚未见报道。在这项研究中，我们发现人类妊娠早期蜕膜中含有丰富的CD4(+)CD25(Bright)T细胞，高水平表达CD152(CTLA-4)。CD4(+)CD25(Bright)T细胞通过抗CD3刺激介导对自体T细胞增殖的有效抑制。此外，这些细胞以剂量依赖的方式抑制自体CD4(+)CD25(-)T细胞的增殖。蜕膜CD4(+)CD25(+)T细胞的这种抑制功能需要细胞与细胞之间的接触。自然流产患者蜕膜组织中CD4(+)CD25(Bright)T细胞的比例明显低于人工流产者。这些结果提示，蜕膜中的CD4(+)CD25(Bright)T细胞参与了母体对妊娠抗原的免疫耐受机制，从而可能有助于维持妊娠。
Human pregnancy represents a situation of semiallograft to maternal host. Therefore, it has been reported that tolerance to the fetal allograft represents a mechanism for maintaining a pregnancy. CD4(+)CD25(bright) regulatory T cells are known to play an important role in the development and maintenance of tolerance in peripheral tissues. However, the potential role of CD4(+)CD25(bright) T cells in maintaining human pregnancy has not been reported. In this study, we show that early human pregnancy decidua contains an abundance of CD4(+)CD25(bright) T cells, which express; CD152(CTLA-4) at a high level. CD4(+)CD25(bright) T cells mediate potent inhibition of autologous T-cell proliferation by anti-CD3 stimulation. Furthermore, these cells inhibit the proliferation of autologous CD4(+)CD25(-) T cells in a dose-dependent fashion. This suppressive function of decidual CD4(+)CD25(+) T cells required cell-to-cell contact. The proportion of decidual CD4(+)CD25(bright) T cells was significantly lower in specimens from spontaneous abortion compared to those from specimens from induced abortions. These results suggest that decidual CD4(+)CD25(bright) T cells contribute to the mechanisms mediating maternal immune tolerance of conceptuts antigens and therefore might contribute to the maintenance of pregnancy.