Digit tip regrowth and differential gene expression in MRL/Mpj, DBA/2, and C57BL/6 mice
Digit tip regrowth and differential gene expression in MRL/Mpj, DBA/2, and C57BL/6 mice
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DOI：
10.1111/j.1524-475x.2007.00216.x
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发表时间：
2007-03-01
影响因子：
2.9
通讯作者：
Baylink, David J.
中科院分区：
文献类型：
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作者：
Chadwick, Robert B.;Bu, Liming;Baylink, David J.
MRL/Mpj小鼠是唯一已知的小鼠品系，可以再生心脏病变，并完全愈合耳拳没有疤痕。这项研究是为了确定MRL小鼠是否也有更大的再生能力，在截肢的指尖。解剖新生MRL小鼠的右爪趾尖，左前爪作为未切割的对照。用于再生长比较的对照是DBA/2和C57 BL/6近交系小鼠品系。在截肢后0、7、14、21和28天采集前爪的连续X射线图像。与DBA和B6小鼠相比，发现MRL小鼠指尖远端再生更快，并且在更大程度上部分和完全地改革指甲（p < 0.05）。我们接下来进行了微阵列表达分析，以确定参与趾尖再生的基因。与第0天对照组织相比，第4天MRL、DBA和B6小鼠中15，000个基因中的400个基因显著差异表达（p < 0.05）。MRL，DBA和B6菌株之间的多重差异被发现在涉及WNT信号通路和转录的基因。我们的结论是，MRL小鼠再生指远端更迅速，部分和完全再生指甲在更大程度上比B6和DBA菌株。这种增强的再生长可能是由于参与生长和发育的基因的菌株特异性表达增加。
MRL/Mpj mice are the only known strain of mouse that can regenerate cardiac lesions and completely heal ear punches without scarring. This study was undertaken to determine if MRL mice also have greater regrowth capabilities in amputated digit tips. Right paw digit tips of neonatal MRL mice were dissected, with the left front paws as uncut controls. Controls used for regrowth comparison were the DBA/2 and C57BL/6 inbred mouse strains. Consecutive x-ray images were captured of front paws at 0, 7, 14, 21, and 28 days postamputation. MRL mouse digit tips were found to distally regrow more quickly and reform nails partially and completely to a greater degree in comparison with DBA and B6 mice (p < 0.05). We next undertook microarray expression analysis to identify the genes involved in digit tip regrowth. Four hundred genes out of 15,000 were significantly differentially expressed (p < 0.05) in MRL, DBA, and B6 mice at day 4 in comparison with day 0 control tissue. Multiple differences between MRL, DBA, and B6 strains were found in genes that are implicated in the WNT signaling pathway and transcription. We conclude that MRL mice regrow digits distally more rapidly and partially and completely regrow nails to a greater degree than B6 and DBA strains. This enhanced regrowth is likely due to strain-specific increased expression of genes involved in growth and development.