FSH acts on the proliferation of type A spermatogonia via Nur77 that increases GDNF expression in the Sertoli cells
FSH acts on the proliferation of type A spermatogonia via Nur77 that increases GDNF expression in the Sertoli cells
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FSH 通过 Nur77 作用于 A 型精原细胞的增殖，从而增加支持细胞中 GDNF 的表达
DOI：
10.1016/j.febslet.2011.06.013
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发表时间：
2011-08-01
期刊：
影响因子：
3.5
通讯作者：
Hu, Ya-Li
中科院分区：
文献类型：
--
作者：
Ding, Li-Jun;Yan, Gui-Jun;Hu, Ya-Li
支持细胞中 GDNF 调节的分子机制仍然很大程度上未知。在本研究中，FSH 诱导小鼠睾丸组织和人胎儿支持细胞中 Nur77 和 GDNF 的表达。此外，FSH 增加了与支持细胞共培养的 A 精原细胞的数量。在其他测定中，观察到 Nur77 直接调节 GDNF 转录。此外，Nur77 的过度表达和 siRNA 介导的 Nur77 敲低影响原代人胎儿支持细胞中 GDNF mRNA 和蛋白质的水平。这些结果表明，FSH诱导的Nur77调节支持细胞中GDNF的表达，从而刺激A精原细胞在体外的增殖。 (C) 2011 年欧洲生化学会联合会。由 Elsevier B.V 出版。保留所有权利。
The molecular mechanism responsible for the regulation of GDNF in Sertoli cells remains largely unknown. In the present study, FSH induced the expression of Nur77 and GDNF in mouse testis tissue and human fetal Sertoli cells. Moreover, FSH increased the number of A spermatogonia co-cultured with Sertoli cells. In the additional assays, Nur77 was observed to directly regulate GDNF transcription. Furthermore, overexpression of Nur77 and siRNA-mediated knockdown of Nur77 affected levels of GDNF mRNA and protein in primary human fetal Sertoli cells. These results indicate that FSH-induced Nur77 regulates the expression of GDNF in Sertoli cells to stimulate the proliferation of A spermatogonia in vitro. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.