PROGNOSTIC-SIGNIFICANCE OF THE 67-KILODALTON LAMININ RECEPTOR EXPRESSION IN HUMAN BREAST CARCINOMAS
PROGNOSTIC-SIGNIFICANCE OF THE 67-KILODALTON LAMININ RECEPTOR EXPRESSION IN HUMAN BREAST CARCINOMAS
复制标题
DOI：
10.1093/jnci/85.5.398
复制
发表时间：
1993-03-03
期刊：
影响因子：
--
通讯作者：
COLNAGHI, MI
中科院分区：
文献类型：
--
作者：
MARTIGNONE, S;MENARD, S;COLNAGHI, MI
背景：67-kd层粘连蛋白受体是一种与层粘连蛋白高亲和力结合的细胞表面蛋白。体外研究表明，这种蛋白质参与了人类肿瘤向浸润性癌症（转移）的进展，但体内研究很少。这些蛋白质的鉴定将允许开发旨在干扰其作用机制的疗法。目的：这项大型回顾性研究旨在研究层粘连蛋白受体分子表达与乳腺癌患者预后因素和总生存率的关系。研究方法：我们使用免疫过氧化物酶技术和MLuC 5单克隆抗体对1160例原发性乳腺癌的石蜡包埋切片进行化学染色，MLuC 5单克隆抗体对67-kd层粘连蛋白受体具有特异性。从1968年1月至1971年12月进行的连续手术中获得标本。淋巴结阴性或受累区域淋巴结仅行手术治疗，腋窝淋巴结阳性者行手术加放疗。在疾病复发前未给予化疗。采用对数秩和法对生存曲线进行统计分析，并根据不同的预后变量建立精算寿命表计算生存率。结果：我们发现层粘连蛋白受体表达与年轻年龄之间有统计学意义的相关性（P
Background: The 67-kd laminin receptor is a cell-surface protein that binds laminin with high affinity. In vitro studies suggest that this protein is involved in the progression of human tumors to invasive cancers (metastasis), but there have been few in vivo studies. Identification of such proteins would allow development of therapies aimed at interfering with their mechanisms of action. Purpose: This large retrospective study was designed to investigate the association of expression of this laminin receptor molecule with established prognostic factors and overall survival in breast carcinoma patients. Methods: We immunohistochemically stained archival paraffin-embedded sections of 1160 primary breast carcinomas, using an immunoperoxidase technique and the MLuC5 monoclonal antibody, which is specific for the 67-kd laminin receptor. Specimens were obtained from consecutive surgeries performed from January 1968 through December 1971. Patients with negative lymph nodes or involved regional nodes had been treated with surgery alone; those with positive axillary nodes had received surgery and radiotherapy. No chemotherapy had been administered until disease recurrence. The statistical analysis was carried out using the logrank method for the survival curves and the actuarial life table to calculate survival rates according to the different prognostic variables. Results: We found statistically significant associations between laminin receptor expression and young age (P