REVERSION TO METHIONINE INDEPENDENCE IN SIMIAN-VIRUS 40-TRANSFORMED HUMAN AND MALIGNANT RAT FIBROBLASTS IS ASSOCIATED WITH ALTERED PLOIDY AND ALTERED PROPERTIES OF TRANSFORMATION
REVERSION TO METHIONINE INDEPENDENCE IN SIMIAN-VIRUS 40-TRANSFORMED HUMAN AND MALIGNANT RAT FIBROBLASTS IS ASSOCIATED WITH ALTERED PLOIDY AND ALTERED PROPERTIES OF TRANSFORMATION
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DOI：
10.1073/pnas.76.3.1313
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
ERBE, RW
中科院分区：
文献类型：
--
作者：
HOFFMAN, RM;JACOBSEN, SJ;ERBE, RW
与正常细胞不同，许多转化细胞和恶性细胞在生长培养基中的甲硫氨酸被其直接前体同型半胱氨酸取代时不生长。来自这些群体的稀有细胞恢复到甲硫氨酸独立性。由SV-40转化的人成纤维细胞和恶性大鼠成纤维细胞的甲硫氨酸非依赖性回复突变体同时回复与转化状态相关的一些性质。在本文所述的13种甲硫氨酸非依赖性回复突变体中，5种表现出增加的锚定依赖性，这反映在甲基纤维素中克隆效率降低; 8种表现出最佳生长所需的血清增加; 8种表现出在含有高血清的培养基中细胞密度降低; 3种显著改变了细胞形态。在这13个中，有8个染色体数目增加。所有检测的细胞系均含有SV-40的免疫学可识别肿瘤抗原。通过选择甲硫氨酸独立性，可以选择异源转化回复突变体，进一步表明改变的甲硫氨酸代谢和致癌转化之间的关系。开发了一种选择转化回复突变体的阳性代谢方法，并使用该方法选择了人转化回复突变体。
Many transformed and malignant cells, unlike normal cells, do not grow when methionine in the growth medium is replaced by its immediate precursor homocysteine. Rare cells from those populations revert to methionine independence. Methionine-independent revertants of both human fibroblasts transformed by SV-40 and malignant rat fibroblasts concomitantly revert for some of the properties associated with the transformed state. Of the 13 methionine-independent revertants described here, 5 showed increased anchorage dependence as reflected by reduced cloning efficiencies in methylcellulose; 8 showed an increased serum requirement for optimal growth; 8 showed decreased cell density in medium containing high serum; and, 3 altered their cell morphology significantly. Of the 13, 8 have increased chromosome numbers. All lines tested contained immunologically identifiable tumor antigen of SV-40. By selecting for methionine independence it is possible to select for heterogeneous transformation revertants, indicating further a relationship between altered methionine metabolism and oncogenic transformation. A positive metabolic method to select for transformation revertants was developed, and its use resulted in selection of human transformation revertants.