THE GYRB GENE-PRODUCT FUNCTIONS IN BOTH INITIATION AND CHAIN POLYMERIZATION OF ESCHERICHIA-COLI CHROMOSOME-REPLICATION - SUPPRESSION OF THE INITIATION DEFICIENCY IN GYRB-TS MUTANTS BY A CLASS OF RPOB MUTATIONS
THE GYRB GENE-PRODUCT FUNCTIONS IN BOTH INITIATION AND CHAIN POLYMERIZATION OF ESCHERICHIA-COLI CHROMOSOME-REPLICATION - SUPPRESSION OF THE INITIATION DEFICIENCY IN GYRB-TS MUTANTS BY A CLASS OF RPOB MUTATIONS
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DOI：
10.1007/bf00334827
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
JONCZYK, P
中科院分区：
文献类型：
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作者：
FILUTOWICZ, M;JONCZYK, P
描述了一类 rpoB 突变，其抑制转移到不允许温度的 gyrB-ts 突变体的复制和转录缺陷。 RNA 聚合酶 (rpoB) 亚基改变对 gyrB 缺陷的补偿作用表明转录是 DNA 旋转酶 B 亚基的主要靶标。一种 gyrB 突变 (gyrB402-ts) 显示在不允许的温度下、体内和用甲苯透化的细胞中染色体伸长缺陷。 gyrB多肽在复制中至少具有双重功能；首先，在转录起始水平，其次，在链聚合水平。
A class of rpoB mutations is described which suppresses replication and transcription deficiency in gyrB-ts mutants shifted to a nonpermissive temperature. The compensatory effect of an altered subunit B of RNA polymerase (rpoB) for the gyrB defect, indicates that transcription is a primary target of the B subunit of DNA gyrase. One gyrB mutation (gyrB402-ts) shows deficiency in chromosome elongation at the nonpermissive temperature, in vivo and in cells permeabilized with toluene. The gyrB polypeptide functions at least dually in replication; first, at the level of transcription initiation and second, at the level of chain polymerization.