H2B ubiquitylation in transcriptional control: a FACT-finding mission
H2B ubiquitylation in transcriptional control: a FACT-finding mission
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DOI：
10.1101/gad.1541507
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发表时间：
2007-04-01
影响因子：
10.5
通讯作者：
Strahl, Brian D.
中科院分区：
文献类型：
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作者：
Laribee, R. Nicholas;Fuchs, Stephen M.;Strahl, Brian D.
10多年前，David Allis的实验室在Cell上发表了两篇开创性的论文（Brownell et al. 1996; Mizzen et al. 1996），证明转录共激活因子Gcn5和TAFII250 （TAF1）可以乙酰化组蛋白，作为其转录激活功能的一部分。这些研究从根本上改变了我们对转录调控的理解，并预示着一个新的研究时代的到来，该研究的重点是了解共价组蛋白修饰如何调节基因转录。最近的一系列研究将一种特殊的修饰——组蛋白H2B单泛素化（H2Bub1）与基因转录周期延长期的调节联系起来。Allis实验室（Tanny et al. 2007）发表在本期《基因与发育》杂志上的一项研究进一步加强了这种联系，该研究表明H2Bub1在体内通过染色质促进RNA聚合酶II （Pol II）的高效延伸。我们将讨论到目前为止建立H2Bub1作为与转录调控相关的修饰的工作，强调当前研究的重要性，以及其他研究，在塑造我们对H2Bub1如何通过染色质景观促进转录延伸的理解。
A little more than 10 years ago, the laboratory of David Allis published two pioneering papers in Cell (Brownell et al. 1996; Mizzen et al. 1996) demonstrating that the transcriptional coactivators Gcn5 and TAFII250 (TAF1) could acetylate histones as part of their transcriptional activating functions. These studies fundamentally altered our understanding of transcriptional regulation and heralded a new era of research that has focused on understanding how covalent histone modifications regulate gene transcription. A recent flurry of studies has linked one modification in particular, histone H2B monoubiquitylation (H2Bub1), to the regulation of the elongation phase of the gene transcription cycle. A study in this issue of Genes & Development from the Allis laboratory (Tanny et al. 2007) further strengthens this connection by showing that H2Bub1 functions in vivo to promote efficient RNA polymerase II (Pol II) elongation through chromatin. We will discuss the work up to this point that establishes H2Bub1 as a modification linked to transcriptional regulation, highlighting the importance of this current study, along with others, in shaping our understanding of how H2Bub1 promotes transcription elongation through a chromatin landscape.